Listing of research core items
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Instrument/equipment
Eppendorf pipettes
We have a variety of Eppendorf micropipettes. Manual and electronic single- and multi-channel pipettes allow for rapid dispensing of simple plate designs.
Located within Catalysis and Separations CoreManisha Skaria
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Instrument/equipment
Light sheet microscope
Miltenyi UM Blaze is a a user-friendly light sheet microscope designed for subcellular 3D imaging of large and multiple samples.
Located within West Campus Imaging Core -
Services
Metabolic flux and enrichment studies using MIMOSA
In this subcore, parameters of cellular and tissue metabolic flux are assessed by incorporation of stable isotope-labeled tracers (e.g., carbon-13, nitrogen-15, or deuterium) into metabolism. The CMC uses the targeted mass isotopomer multi-ordinate spectral analysis (MIMOSA) platform to interpret stable isotope labeling patterns for calculation of rates of discrete steps in glycolytic and mitochondrial metabolism. Such measurements can be used to identify differences in fuel usage (e.g., glycolysis vs. beta-oxidation), and non-oxidative (reductive carboxylation, anaplerosis and cataplerosis) contributions to the TCA cycle.
Located within Chemical Metabolism CoreStephan Siebel, MD, PhD
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Instrument/equipment
NMR
Our NMR instruments include a Bruker 400 MHz broadband probe and an Agilent DD2 600 MHz 3 mm cold probe.
Located within West Campus Analytical Core -
Instrument/equipment
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Instrument/equipment
Other microscopes
Learn about our Leica DMi8 epifluorescence, Leica Thunder Imager 3D epifluorescence, Leica LMD7000 laser microdissection, and Leica M125 stereo microscopes and Thorlabs optical tweezers system.
Located within West Campus Imaging Core -
Services
Quantitative targeted analysis
Identification and quantification of key metabolites of central carbon metabolism.
Located within Chemical Metabolism CoreStephan Siebel, MD, PhD
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Instrument/equipment
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Services
Islet isolation and function
In this subcore, our technical expertise in isolating rodent islets including rats, mice, and most recently ground squirrel from the pancreas ex vivo allows researchers to study the intact islets for their studies or to proceed with substrate-stimulated insulin secretion functional studies using our BioRep Technologies Islet Perfusion Systems (8-channel and 12-channel). For non-rodent islet studies, human donor islet procurement assistance as well as functional studies are also offered.
Located within Chemical Metabolism CoreRebecca Cardone, MS
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Instrument/equipment
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Instrument/equipment
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Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration (OCR) and acidification (ECAR) are assessed with the Agilent Seahorse XF Pro 96. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. Changes in acidification are also observed simultaneously. An integrated Agilent BioTek Cytation5 imager correlates cell number via Hoechst nuclear stain to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS