Listing of research core items
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Services
Quantitative targeted analysis
Identification and quantification of key metabolites of central carbon metabolism.
Located within Chemical Metabolism CoreStephan Siebel, MD, PhD
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Instrument/equipment
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Services
Islet isolation and function
In this subcore, our technical expertise in isolating rodent islets including rats, mice, and most recently ground squirrel from the pancreas ex vivo allows researchers to study the intact islets for their studies or to proceed with substrate-stimulated insulin secretion functional studies using our BioRep Technologies Islet Perfusion Systems (8-channel and 12-channel). For non-rodent islet studies, human donor islet procurement assistance as well as functional studies are also offered.
Located within Chemical Metabolism CoreRebecca Cardone, MS
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Instrument/equipment
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Instrument/equipment
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Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration (OCR) and acidification (ECAR) are assessed with the Agilent Seahorse XF Pro 96. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. Changes in acidification are also observed simultaneously. An integrated Agilent BioTek Cytation5 imager correlates cell number via Hoechst nuclear stain to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS
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Instrument/equipment
Abberior MIRAVA Polyscope
Super-resolution confocal laser scanning microscope with STED and MINFLUX.
Located within Wu Tsai Institute Microscopy Shared FacilityLin Shao, PhD
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Core/facility
Aberration-Corrected Electron Microscopy (ACEM) Core
Our core's focus is cutting-edge and high-throughput capability in electron microscopy techniques, including TEM, SEM, and FIB.
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Core/facility
Advanced Instrumentation Development Center (AIDC)
Our mission stands at the nexus between hardware, computing, and data science.
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Core/facility
Advanced Prototyping Center at Wright Lab
The Advanced Prototyping Center is a fabrication and design support facility to help Yale researchers with custom instrumentation.
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Instrument/equipment
Electronics
We are building an electronics shop to help researchers with printed circuit board design and fabrication, analog and digital hardware development, and advanced instrumentation electronics spanning FPGA-based designs, low-noise circuits, RF/microwave hardware, mixed-signal systems, and embedded systems.
Located within Advanced Instrumentation Development Center (AIDC) -
Instrument/equipment
Professional machining
We help researchers with expert conceptual design, engineering, prototyping, and custom manufacture of scientific equipment. Note: The machine shop has moved to SCL 22 due to the construction of upper Science Hill.
Located within Advanced Instrumentation Development Center (AIDC)