Keck DNA Sequencing Facility
9 AM - 5 PM
Announcement:
We are closed on Monday 1/20/2025 in observance of the University holiday.
About Keck DNA Sequencing
The Keck DNA Sequencing Facility provides competitive and timely DNA sequencing in an efficient and cost-effective manner. Sequencing is carried out on our Applied Biosystems 3730xL DNA Analyzers, along with Applied Biosystems Big Dye chemistries.
Services include:
Available to Yale researchers & external researchers
Create a Seq account
Users need a sequencing account to submit samples. Create yours here.
Sample submission
Standard turnaround time is 1 business day*. (*CLA turnaround time is 5 business days.) Yale users will receive priority over non-Yale users. If your samples are RUSH samples, please make us aware of this before we receive your submission.
Sample submission formContacts
Mailing address:
Keck DNA Sequencing Lab
Attn: Jaime Heltke
300 George Street
2nd Floor, Room 2127
New Haven, CT 06511
Staff
Rates
As of 7/1/2024
| Service | Yale user | External for-profit user | External non-profit user |
| Consulting, per hour | $100.00 | $150.00 | $150.00 |
| Fragment analysis, 96-well plate, per well (24 minimum) | $1.40 | $2.00 | $1.75 |
| Human cell line authentication (CLA) | $75.00 | $100.00 | $100.00 |
| PCR purification, per 96-well plate | $384.00 | $528.00 | $528.00 |
| Re-plate samples, incorrect plasticware | $10.00 | $15.00 | $15.00 |
| Sanger sequencing, single tube | $3.50 | $5.00 | $4.85 |
| Sanger sequencing, 96-well plate, per well (48 minimum) | $1.99 | $2.75 | $2.75 |
| Sanger sequencing, 384-well plate, per well | $1.85 | $2.50 | $2.50 |
| Sanger sequencing difficult template surcharge, per sample/well | $2.35 | $3.15 | $3.15 |
| TapeStation QC, per sample | $20.00 | $27.75 | $27.50 |
Services
-
DNA/RNA QC analysis service
QC using the Agilent Tapestation 2200 allows researchers to analyze the quantity and size of DNA or RNA while using only a few microliters of precious sample.
Available to Yale researchers & external researchers -
Fragment analysis
Our 3730XL DNA Analyzer uses fluorescence-based detection to determine the size of DNA fragments.
Available to Yale researchers & external researchers -
Human cell line authentication
Using a cell line authentication service can help to establish the identity of a cell line or confirm that it remains uncontaminated. With the use of the CLA Identifiler Direct Kit, we will perform STR genotyping by fragment analysis. This STR profile will be the key to authenticating the cell line.
Available to Yale researchers & external researchers -
PCR purification
PCR purification for 96-well plate format is available to experienced users who are confident in their methodology for PCR and primer preparation.
Available to Yale researchers & external researchers -
Sanger sequencing
Costs start as low as $1.99/well and $3.50/tube submission. Sequencing performed using Thermo Fisher technologies and can be submitted in tube format or 96-well plate format.
Available to Yale researchers & external researchers
Yale user resources
Non-Yale user resources
Sanger sequencing template prep instructions
2uL 4uM Primer + DNA(see table) + (sterile H2O, if necessary) = 18uL
Product type Product size DNA (ng) Ordering notes PCR 100-300 bp 40-50 *Can be ordered with Difficult Template or shRNA protocol 300-500 bp 40-160 500-1000 bp 500 Plasmid 1000-1500 Single-stranded M13 or phagemic DNA 200-300 Order as single-stranded BAC and phage lambda DNA 4000 Order as BAC - Each reaction should include 2uL of a 4uM Primer. One primer per reaction.
- The total volume should be 18uL. If extra volume needed, use sterile H2O.
Keck HIPAA policies
The Keck Facility will not accept samples containing protected health information (PHI) or electronic protected health information (ePHI) that is covered under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any individually identifiable health information associated with a sample must be removed by the user before the sample will be accepted. Learn more about Keck HIPAA Policies for Sample Submission.
Publication acknowledgment
If research supported by this research core results in publication, please acknowledge this support by including the following text in your publication(s):
"We thank the Keck DNA Sequencing Facility at Yale for their assistance with _________ service.”
Index of pages
| Webpage | Topic |
| Freeware | Software library |
| Sample drop-off locations | General information |
| Shipping your samples | General information |
| Contact us | General information |
| Plasticware requirements | General information |
| Direct sequencing of PCR products | Sanger Sequencing |
| Plasmid DNA template preparation | Sanger Sequencing |
| Primer selection guidelines | Sanger Sequencing |
| Plate sequencing | Sanger Sequencing |
| Tube sequencing | Sanger Sequencing |
| Sanger Sequencing Troubleshooting Guide | Troubleshooting |
| Why did my sequence fail? | Troubleshooting |
| QC analysis troubleshooting guide | Troubleshooting |
-
Core/facility
Yale Center for Genome Analysis (YCGA)
The Yale Center for Genome Analysis is a full-service facility dedicated to providing RNA expression profiling, DNA genotyping, and high-throughput sequencing using state-of-the-art technologies.
Primary contact
-
Core/facility
Yale Center for Molecular Discovery
We provide resources for assay development and high-throughput screening with small molecules and arrayed siRNA and sgRNA/CRISPR libraries in biochemical, cell-based and image-based assays (primarily 384-well format).
-
Core/facility
Chemical Metabolism Core
Previously called the Islet, Oxygen consumption, Mass Isotopomer flux Core (IOMIC), the Chemical Metabolism Core offers targeted and untargeted metabolomics, stable isotope based metabolic flux analysis, quantitative metabolic panels of central carbon metabolism and adjacent pathways, measurements of cellular bioenergetics, islet isolation and functional studies. Services include study assistance and data analysis.