Plasticware requirements
Keck DNA Sequencing requires users to submit in plastics that are compatible with our robotics. Most of these plastics are located in Yale stockrooms or can be ordered on SciQuest.
Keck DNA Sequencing
PLEASE NOTE!! Our facility will be closed on Thursday, November 28 & Friday, November 29, 2024 in observance of the University Holiday. Please have your samples in the pickup buckets by 9:30 on Wednesday, November 27 to ensure processing before the break. You may also bring them in person before 10 AM. If you have any questions, please contact dnaseq@yale.edu.
Sample submissionAcceptable tube types
For Sanger sequencing:
Tube submissions must be freestanding with a 0.5ml interior that is not located at the bottom of the tube.
Individual tube reactions submitted in any other tube type will not be accepted. We will ask you to resubmit or we will charge a tube transfer fee. Here are some examples of acceptable tubes types:
DOT Scientific screw cap tubes. 914-NC (tubes) and 503-SC (caps)
Genesee Scientific: 21-258 tubes with caps
USA Scientific: 1405-9700 tubes with caps (any color)
VWR Labcon: 76417-162 tubes with customizable caps, and 89004-284 tubes with green caps
For TapeStation QC:
Any tube type and size is acceptable, as long as the samples are clearly labeled.
Acceptable 96-well plate types
For Sanger sequencing and PCR purification:
All high-volume plate submissions must be submitted in semi-skirted 2 mL 96-well plates with clear wells, and sealed with strip caps or sealing film/foil. If submitted incorrectly, we will require samples transfer to a compatible plate. There is a fee associated with the sample transfer.
- Eppendorf Twin Tec plates part numbers: 030129326, 951020303, 0030129610, 0030133366; strip caps part numbers: 0030124839, 0030124847, 951022089
- ABI MicroAmp Optical plates, part numbers: N801-0560, Strip Caps part #N801-0534
- DOT Scientific PCR plates, plate part numbers: 351-PCR, 351LP-PCR, 352-PCR, 354-PCR and 650-PCR, Strip Caps part numbers: #405-12PCR, #400-12PCR; Sealing Foil options #T392.
- USA Scientific TempPlate #1402-9596
For fragment analysis:
All samples must be submitted in a (non-fully-skirted) 96-well optical plate with clear 2 mL wells. If using plates with thicker plastic, the angled corner must be in A12. Fast plates will not work with our instrumentation and will require samples transfer to a compatible plate. There is a fee associated with the sample transfer. (See RATES section.)
These are some examples of acceptable plate types:
- ABI MicroAmp Optical plates, part #N801-0560.
- USA Scientific TempPlate, part #1402-9596
Thomas Scientific Non-Skirted PCR plates, part #1148A37
DOT plates
351-PCR
352-PCR
Acceptable for sanger sequencing & PCR purification
BioDot pure 96 Well ABI style fast PCR plate, half skirted, low profile, raised…
353-PCR
Acceptable for all services
Available in Yale stockrooms
DOT plates
BioDot Pure 96 Well Non Skirted PCR Plate
370-LP
Acceptable for sanger sequencing & PCR purification
Available in Yale stockrooms
455-PCR
Acceptable for sanger sequencing & PCR purification
Available in Yale stockrooms
BioDot Pure Ultra Thin Wall 96 Well Half Skirted Low Profile PCR Plate
Eppendorf plates
Catalog No. 0030129326, Catalog No. 951020303
Acceptable for sanger sequencing submission, PCR purification
Catalog No. 0030129610
Acceptable for sanger sequencing, PCR purification
Catalog No. 0030133366
Acceptable for all services
PLEASE NOTE!! Our facility will be closed on Thursday, November 28 & Friday, November 29, 2024 in observance of the University Holiday. Please have your samples in the pickup buckets by 9:30 on Wednesday, November 27 to ensure processing before the break.
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