Plasticware requirements

Keck DNA Sequencing requires users to submit in plastics that are compatible with our robotics. Most of these plastics are located in Yale stockrooms or can be ordered on SciQuest.

Keck DNA Sequencing

PLEASE NOTE!! Our facility will be closed on Thursday, November 28 & Friday, November 29, 2024 in observance of the University Holiday. Please have your samples in the pickup buckets by 9:30 on Wednesday, November 27 to ensure processing before the break. You may also bring them in person before 10 AM. If you have any questions, please contact dnaseq@yale.edu.

Sample submission

Acceptable tube types

For Sanger sequencing:

Tube submissions must be freestanding with a 0.5ml interior that is not located at the bottom of the tube. 

DOT Scientific 914-NC

Individual tube reactions submitted in any other tube type will not be accepted. We will ask you to resubmit or we will charge a tube transfer fee. Here are some examples of acceptable tubes types:

DOT Scientific screw cap tubes.  914-NC (tubes) and 503-SC (caps)

Genesee Scientific: 21-258 tubes with caps

USA Scientific: 1405-9700 tubes with caps (any color)

VWR Labcon: 76417-162 tubes with customizable caps, and 89004-284 tubes with green caps

For TapeStation QC:

Any tube type and size is acceptable, as long as the samples are clearly labeled.

Acceptable 96-well plate types

For Sanger sequencing and PCR purification:

All high-volume plate submissions must be submitted in semi-skirted 2 mL 96-well plates with clear wells, and sealed with strip caps or sealing film/foil. If submitted incorrectly, we will require samples transfer to a compatible plate. There is a fee associated with the sample transfer.

  • Eppendorf Twin Tec plates part numbers: 030129326, 951020303, 0030129610, 0030133366; strip caps part numbers: 0030124839, 0030124847, 951022089
  • ABI MicroAmp Optical plates, part numbers: N801-0560, Strip Caps part #N801-0534
  • DOT Scientific PCR plates, plate part numbers: 351-PCR, 351LP-PCR, 352-PCR, 354-PCR and 650-PCR, Strip Caps part numbers: #405-12PCR, #400-12PCR; Sealing Foil options #T392.
  • USA Scientific TempPlate #1402-9596
For fragment analysis:

All samples must be submitted in a (non-fully-skirted) 96-well optical plate with clear 2 mL wells. If using plates with thicker plastic, the angled corner must be in A12. Fast plates will not work with our instrumentation and will require samples transfer to a compatible plate. There is a fee associated with the sample transfer. (See RATES section.)
These are some examples of acceptable plate types:

  • ABI MicroAmp Optical plates, part #N801-0560.
  • USA Scientific TempPlate, part #1402-9596
  • Thomas Scientific Non-Skirted PCR plates, part #1148A37

    Angled corner in A12 of plate

DOT plates

Natural, raised top

351-PCR

Acceptable for all services

Available in Yale stockrooms

BioDot pure 96 well half skirted PCR plate

Low profile, clear

352-PCR

BioDot pure 96 well semi skirted standard well PCR plate

353-PCR

Acceptable for all services

Available in Yale stockrooms

BioDot pure 96 well semi skirted standard well PCR plate

DOT plates

BioDot Pure 96 Well Non Skirted PCR Plate

BioDot Pure 96 Well Non Skirted PCR Plate

Acceptable for all services

Available in Yale stockrooms

BioDot Pure 96 Well Non Skirted PCR Plate

370-LP

Acceptable for sanger sequencing & PCR purification

Available in Yale stockrooms

BioDot Pure 96 Well Non Skirted Low Profile PCR Plate,

BioDot Pure Ultra Thin Wall 96 Well Half Skirted Low Profile PCR Plate

455-PCR

Acceptable for sanger sequencing & PCR purification

Available in Yale stockrooms

BioDot Pure Ultra Thin Wall 96 Well Half Skirted Low Profile PCR Plate

Eppendorf plates

Eppendorf twin.tec® microbiology PCR Plate 96

Catalog No. 0030129326, Catalog No. 951020303

Acceptable for sanger sequencing submission, PCR purification

Eppendorf twin.tec® microbiology PCR Plate 96

Eppendorf twin.tec® PCR Plate 96

Catalog No. 0030129610

Acceptable for sanger sequencing, PCR purification

Eppendorf twin.tec® PCR Plate 96

Eppendorf twin.tec® PCR Plate 96

Catalog No. 0030133366

Acceptable for all services

Eppendorf twin.tec® PCR Plate 96

PLEASE NOTE!! Our facility will be closed on Thursday, November 28 & Friday, November 29, 2024 in observance of the University Holiday. Please have your samples in the pickup buckets by 9:30 on Wednesday, November 27 to ensure processing before the break.

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