Chemical Metabolism Core

The Chemical Metabolism Core (CMC) offers a wide variety of metabolic studies, including targeted and untargeted metabolomics, stable isotope based metabolic flux analysis of central carbon metabolism, quantitative targeted panels of key metabolic intermediates and pathways, measurement of cellular bioenergetics and cellular respiration as well as functional islet cell studies and islet cell isolation and procurement. Each of these services can be ordered as focused studies of mitochondrial biochemistry and cell metabolism or can be integrated for investigation of global metabolism. Understanding metabolism goes beyond data collection. Our team has extensive experience with the CMC platforms and is available to assist in experimental design, the development of new assays, and interpretation of data for collaboration or consultation fees. 

Current services offered

• Cell, tissue, and plasma metabolomics (~650 targeted and ~2000 untargeted metabolites)
• Integrated Omics (IntOmics) analysis combining RNAseq enzyme transcripts with metabolomic data on an optimized metabolic network
• Cell and tissue stable isotope metabolic flux analysis
• Targeted metabolite concentrations
• Cell respirometry
• Pancreatic islet isolation (rodent) and islet functional studies, including islet perfusion and static incubation studies in rodent and human islets

Instruments and equipment

• Sciex 6600+ QToF SelexION LC-MS/MS with Selexion (UHPLC and gas generator)
• Sciex 6500+ QQQ SelexION LC-MS/MS with Selexion (UHPLC and gas generator)
• Sciex 5500+ QTrap Selexion LC-MS/MS with Selexion (UHPLC and gas generator)
• Agilent Seahorse XF Analyzer Pro 96
• BioTek Cytation5 Imager for Respirometry Normalization (Hoechst nuclear imaging for cell number)
• Biorep Technologies Islet Perfusion Systems (8-channel)
• Biorep Technologies Islet Perfusion Systems (12-channel)
• MD6000 Alabama R&D Tissue Slicer with MD2200 Tissue Embedding Unit
• Biorep PERI-PSC-001 - TISSUE SLICE CHAMBERS

Acknowledgment and authorship

The core provides raw and normalized data as well as advanced analysis (global metabolomics only) for additional fees. If data provided by the core is used in publications, grant applications, and/or presentations then a standard acknowledgment of the core should be added:

“[Name of study] was designed and performed by the Chemical Metabolism Core at Yale University. We thank Yale University's Chemical Metabolism Core, especially [staff name], for their assistance with [technique/technology] and contribution to this project.”

Data interpretation in the context of a client’s projects is not an integral part of the core's services. If those services are desired and provided by one of the core’s scientists, then that scientist should be considered an author on the resultant publication(s) as per authorship guidelines published by the NIH, which include one or more of the following criteria:

• Conception or design of the project
• Original ideas or critical input
• Interpretation of data
• Drafting or revising the article for intellectual content
• Writing a portion of the paper, not just the materials and methods section
• Final approval of the version to be published