Quantitative targeted analysis

In addition to relative metabolic flux analysis through MIMOSA, our targeted metabolomics platform offers identification and quantification of key metabolites of central carbon metabolism, such as glycolysis/gluconeogenesis, Krebs cycle, along with select intermediates of ketone and creatine metabolism (see list below). 

Metabolite identification is done by retention time and unique precursor to product ion relationships. Absolute quantitation of sample analytes is performed through external calibration using a standard curve for each metabolite.

Additional analysis offered includes readouts of redox potential and energy balance through ratiometric investigation of metabolites involved in reductive and oxidative reactions and/or transfer of phosphate groups from high-energy metabolites.

The list of targeted metabolites is constantly evolving and the Chemical Metabolism Core offers custom method development of metabolites of interest for research groups.