Cellular and mitochondrial respiration and bioenergetics
Rebecca Cardone, MS
About the instrument
In this subcore, cellular and mitochondrial respiration are assessed with the Agilent Seahorse XF Pro.
The XF Analyzer is the first and only commercially available instrument for scientific research that simultaneously measures in real-time and in live cells the two major energy pathways of the cell: mitochondrial respiration and glycolysis. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. An integrated Agilent BioTek Cytation5 imager correlates cell number to respiration for more accurate normalization of respiration.
Since 2007 when its patent was issued, the XF Analyzer has been used in laboratories worldwide to more rapidly and more accurately obtain real-time measurements of cellular respiration, oxidative phosphorylation, motility, and transport pathways. Such measurements would be invaluable in gaining insights into the mechanisms of many diseases, including cancer, that are linked to metabolic dysfunction or reprogramming. Please follow the links for more information about Seahorse XF Analyzer, how to run an assay, and measuring mitochondrial respiration.
Using Seahorse XF Analyzers, we can readily obtain novel and important data from live cells in real time, thereby providing a greater understanding of cell metabolism and enabling new advancements in life science research.
Learn more about our supporting centers, Yale Cancer Center and Diabetes Research Center.
Available to Yale researchers & external researchers
Core websiteTurnaround time
Please note, the typical turnaround time for data from these services is on average 2 to 3 months but can vary depending on laboratory work load and the complexity of the study.
Sample submission
Rates
Seahorse study
- Internal: $450/4-hour block
- External: Please request quote
Study assistance
- Internal: $134/hour
- External: Please request quote
Additional study assistance is required for full-service oxygen consumption and extracellular acidification studies, Hoechst imaging normalization, and data analysis based on a per-hour fee.
All consumables for running the seahorse assays are provided by the individual user and not provided by IOMIC core. Each user is responsible for procuring these items before they sign up for their studies. The Seahorse XFe96 cartridge and cell plates (Seahorse XFe96 FluxPaks) as well as other reagents that are specific to the cell type and experiment must be provided by the user including running assay media, drugs for injections, and prepared injection solutions. For full-service studies with data normalization, an extra charge for Hoechst stain and study assistance time will be added to the total cost.
For more information on planning and scheduling your studies, please email Rebecca Cardone.
Further information
Supporting centers
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Services
Global metabolomics and integrative -omics (intOmics)
Metabolomics provides screening of targeted (~650) and untargeted (~2500) metabolites. Options for more detailed analyses include volcano plots, pathway enrichment and KEGG network optimization data. Metabolomic data can be integrated with (user provided) RNAseq using a metabolomics network analysis platform (IntOmix), for an optimized pathway analysis of relationships between metabolites and enzyme transcripts.
Located within Chemical Metabolism CoreStephan Siebel, MD, PhD
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Services
Quantitative targeted analysis
Identification and quantification of key metabolites of central carbon metabolism.
Located within Chemical Metabolism CoreStephan Siebel, MD, PhD
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Instrument/equipment
Invitrogen Attune NXT Cell Analyzer
Analyze cells from tubes and plates with 4 lasers (violet, blue, yellow/green, red) and 14 detectors (6 violet, 2 blue, 3 yellow, 3 red).
Located within West Campus Flow Cytometry Facility