Technologies

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Q-Exactive HF-X
Orbitrap Fusion
Q-Exactive Plus
4000 QTRAP
H-Class UPLC
 

UPLC ESI Q-Exactive HF-X MS system for global proteome profiling and quantitation: A high-resolution quadrupole-orbitrap hybrid mass spectrometer from Thermo Fisher Scientific. [Back to top]

• Can reach scan speeds up to 40 Hz at a resolution of 240,000 at m/z 200.
• Equipped with a high-capacity transfer tube, electrodynamic ion funnel, and high-performance quadrupole for improved sensitivity and selection.
• Primarily designated for large-scale, bottom-up proteomic analyses such as data-dependent acquisition (DDA) and data-independent acquisition (DIA) in our facility.
• Can also be used for top-down proteomics (mass range 50-6000 m/z) as well as identification and quantitation of post-translational modifications. 

Nano-UPLC ESI Orbitrap Fusion MS systems for global and targeted proteome profiling and quantitation: A high-resolution Orbitrap Tribrid mass spectrometer from Thermo Fisher Scientific. [Back to top]

• Dual-pressure linear ion trap for CID and ETD fragmentation.
• Progressively spaced stacked-ring S-lens for efficient tight ion beam focusing.
• Active ion beam guide for improved ion transfer.
• Higher-energy collisional dissociation cell for storage and fragmentation.
• Can reach scan speeds up to ~18 Hz, obtain resolution up to 450,000 at m/z 200, provide dynamic range or >5000 within a single scan, and an upper m/z limit of 6000 in the Orbitrap.
• Primarily designated for large-scale, bottom-up proteomic analyses, such as:
  • Data-dependent acquisition (DDA) and data-independent acquisition (DIA)
  • Label-free quantitation (LFQ)
  • Parallel-reaction monitoring (PRM)
  • Labeled (tandem mass tag [TMT]) proteomics approaches
• Can also be used for top-down proteomics as well as identification and quantitation of post-translational modifications. 

Nano-UPLC ESI Q-Exactive Plus MS systems for global proteome profiling and quantitation: A high-resolution quadrupole-orbitrap Hybrid mass spectrometer from Thermo Fisher Scientific. [Back to top]

• Can reach scan speeds up to 20 Hz at a resolution of 240,000 at m/z 400.
• High-capacity transfer tube, electrodynamic ion funnel, and high-performance quadrupole for improved sensitivity and selection.
• Primarily designated for bottom-up proteomic analyses such as data-dependent acquisition (DDA) to identify proteins and protein posttranslational modification from gel bands, immunoprecipitates, cell lysate, etc.
• Can also be used for quantitative proteomics such as SILAC and LFQ workflows. 

UPLC 4000 QTRAP MS system for targeted small molecule quantitation: This triple quadrupole mass spectrometer is primarily used for our small-molecule targeted quantitation workflow. [Back to top]

• Though very dependent on the analyte of interest and its ionization efficiency, this multiple reaction monitoring-based system has been utilized to detect compounds/ion with concentration range >5ng/mL.
• System is coupled to an Agilent 1200 HPLC for online separation prior to the MS detection.

Waters H-Class UPLC for offline fractionation of proteomic and small molecule samples: An ultrahigh-pressure liquid chromatography (UPLC) system for offline peptides, proteins, and small molecule separation. [Back to top]

• Two separate temperature-controlled column compartments with capacity for two columns hooked up separately or serially.
• Plate or tube fraction collector for downstream analyses of the fractionated samples.
• Photodiode array (UV) detector connected for up to 4 UV channels recording/detection.
• Primarily used for TMT multiplex workflow, where offline high pH 1st-dimensional reverse phase separation and subsequent fraction collection is implemented to enhance proteome depth of coverage.
• Also utilized by the core/users for small-molecule separation to determine active components in a reaction mixture.
  
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