Keck Mass Spectrometry & Proteomics Resource
About the core
The Keck Mass Spectrometry (MS) & Proteomics Resource at the Yale School of Medicine provides efficient and cost-effective proteomics and small-molecule mass spectrometry-based services. Besides our many standard operating procedures, we also bring state-of-the-art mass spectrometry-based biotechnologies in response to the input and requests of our users. These technologies are developed and implemented with a broad spectrum of mass spectrometry-based techniques and protein/peptide/small-molecule chemistries to separate, characterize, profile, and quantify analytes from and array of complex biological samples.
Services in brief
- Protein ID from gel bands or immunoprecipitated workflow
- Protein ID from complex solution (e.g. tissue, cells, biological fluids, etc.)
- Protein post-translational modification (PTM) identification
- Protein profiling and quantitation (label-free quantitation, SILAC)
- High-resolution (HR) MS for small molecule and structural elucidation
- Targeted proteomics (PRM)
- Targeted small molecule quantitation (MRM)
Instruments
- ACQUITY M-Class UPLC coupled to Q-Exactive HFX mass spectrometer
- NanoACQUITY UPLC coupled to Q-Exactive Plus mass spectrometer
- NanoACQUITY UPLC coupled to Orbitrap Fusion Tribrid mass spectrometer
- Agilent 1200 HPLC coupled to 4000 QTRAP Triple quadrupole mass spectrometer
- ACQUITY H-Class UPLC with fraction collector
Services in detail
Protein/peptide fractionation
- Reverse-phase HPLC (high or low pH)
- Strong cation exchange HPLC
Post-translational modifications (PTMs)
- Phosphorylation & phosphoproteomics
- Phosphopeptides enrichment
- Phosphorylation site identification
- Global phosphor protein identification
- Palmitoylation
- Ubiquitination/SUMOylation
- Acetylation, methylation, and dimethylation
- Other chemical modifications (e.g., covalently bound irreversible molecule)
Protein identification
- Bottom-up LC MS/MS
- Multi-dimensional protein identification technology (MudPIT)
- IP and Co-IP protein identification
- Cross-linked protein identification
- Identification of proximity-labeled proteins (e.g., BioID)
- Contamination protein ID
Protein profiling
- Offline protein/peptide fractionation
- Stable isotopic labeling by amino acids in cell culture (SILAC)
- Label-free quantitation (LFQ)
- Multi-dimensional protein identification technology (MudPIT)
- TMT
- Data-independent acquisition (DIA)
Small-molecules quantitation
- MRM (multiple reaction monitoring)
- Biocrates p180 assay
- PK study
- Drug metabolite quantitation
Targeted proteomics
- PRM (parallel reaction monitoring)
- Data-independent acquisition (DIA) for cerebrospinal fluid (CSF)
Available to Yale researchers & external researchers
Core websiteContacts
Shipping address:
Keck MS & Proteomics Resource
Yale School of Medicine
300 George Street
Ground floor, room G001
New Haven, CT 06511
Director
Faculty Director
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Primary contact
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