Keck Oligo Synthesis Resource
We provide high-quality, timely DNA and RNA synthesis in individual tubes or plates.
About the core
The Keck Oligonucleotide Synthesis facility carries out DNA and RNA synthesis on our Dr. Oligo 192 and Applied Biosystems 394 synthesizers. We routinely perform a wide variety of complex specialty syntheses, emphasizing quality and efficiency.
Services
- Modifications
- RNA & 2’-O-methyl RNA
- Mixed-base/doped oligos
- Trityl ON oligos
- UltraMild synthesis
- Purification
- Protocols
- Custom cloning with the BioXp™3200 System; dsDNA fragments from 350-1.8 kb can be cloned into the vector of your choice
- 25 nmole service
- Free desalting of normal, un-modified DNA oligos <120 bases synthesized at the 25 and 40 nmole scales
- Large-scale synthesis
Contacts
Primary contact
Faculty Director
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Core/Facility
Yale Center for Genome Analysis (YCGA)
The Yale Center for Genome Analysis is a full-service facility dedicated to providing RNA expression profiling, DNA genotyping, and high-throughput sequencing using state-of-the-art technologies.
Primary contact
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Core/Facility
Yale Center for Molecular Discovery
We provide resources for assay development and high-throughput screening with small molecules and arrayed siRNA and sgRNA/CRISPR libraries in biochemical, cell-based and image-based assays (primarily 384-well format).
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Core/Facility
Chemical Metabolism Core
Previously called the Islet, Oxygen consumption, Mass Isotopomer flux Core (IOMIC), the Chemical Metabolism Core measures islet function, oxygen consumption, and cellular metabolic flux. Services include study assistance and data analysis.