Modifier pricing

There are three factors in determining the cost of a modified oligo:

The synthesis cycle to be used


Determining the cost of a modified oligo begins with selecting the scale of the synthesis, then determining the appropriate per-residue charges.

  • A normal DNA per-residue charge is used for the modified residues unless those modifiers are RNA or 2'-O- methyl RNA.

Please see our price schedule and Oligo Cost Calculator for charges. 

Most of our specialty reagents are ordered from Glen Research. Glen offers only 0.2 micromole and 1.0 micromole columns for initiating syntheses with 3' modified residues. 

Contact oligos@yale.edu for a quote.

The number of additions per bottle


Glen generally offers specialty reagents in 50 micromole, 100 micromole, and 0.25 gram bottles. The number of additions available per bottle varies with each cycle and is displayed below:

50 micromole bottle

LV 40 nm cycle

7 additions

LV 0.2 µm cycle

5 additions

Normal 0.2 µm cycle

2 additions

Normal 1.0 µm cycle

2 additions

100 micromole bottle

LV 40 nm cycle

16 additions

LV 0.2 µm cycle

14 additions

Normal 0.2 µm cycle

5 additions

Normal 1.0 µm cycle

5 additions

0.25 g Bottle

The number of additions per 0.25 g bottle varies with molecular weight. E-mail us at oligos@yale.edu for more information.

Pre-mixed 0.25 g "N" amidite (3.0ml) 
or 3ml doped mixture

LV 40 nm cycle

48 additions

LV 0.2 µm cycle

45 additions

Normal 0.2 µm cycle

17 additions

Normal 1.0 µm cycle:

17 additions

The prices of specialty reagents


In addition to the per-residue charges, the Glen Research or other company's list price + a surcharge for the specialty amidites and/or columns along with any ancillary reagents will be applied.

To eliminate issues of stability of the unincorporated monomers, we special-order the reagent for your oligos and discard any leftovers. We will work with you to group your orders so as to minimize your cost by maximizing the utilization of expensive monomers. If you prefer, we will work our way down a list of oligos until a specialty reagent is exhausted.

Generally, we will incorporate ANY commercially available modifier, not just those listed below, as long as it is non-radioactive and particulate-free. To avoid issues of improper storage and handling, we recommend allowing us to order the modified phosphoramidite/column. However, if you provide us with a commercially available phosphoramidite/column, our standard pricing will apply. If you would like to provide us with a phosphoramidite/column that is not commercially available, a surcharge for each different modification will apply. This surcharge covers the additional labor costs of modified phosphoramidite/column handling and storage, synthesizer programming, oligo processing and e-mail correspondence. Also, since we are not supplying the modified phosphoramidite/column, our usual quality guarantee does not apply.

The following is a list of commonly ordered specialty reagents and columns. 

Please contact oligos@yale.edu for pricing.

  • Trimer phosphoramidites
  • UltraMild synthesis
  • Inosine
  • Chemical phosphorylation
  • Primary amines and thiols
  • Dyes
  • Halogenated deoxy and ribonucleotides
  • Spacers
  • Biotin
  • Reverse synthesis
  • Branches
  • Chain terminators
  • Cross-linking