Islet isolation and function
Rebecca Cardone, MS
About the service
The islet cell biology component of the core offers isolation of islets, high-throughput parallel perfusion of islets, and dynamic studies of hormone secretion. Learn more about the Diabetes Research Center core.
Available to Yale researchers & external researchers
Core WebsiteTurnaround time
The typical turnaround time for data from these services is on average 2 to 3 months for the initial analysis but can vary depending on laboratory workload and the complexity of the study.
Sample submission
Rates
Costs are per plate.
Rodent islet isolation(s)
Single isolation
- Internal: $804
- External: Please request quote
Price includes one isolation condition (1-2 control rats or 4 mice of same genotype) for one pooled batch of islets.
Multiple isolations
- Internal: $1,005
- External: Please request quote
Price includes two maximum isolations per day (2-4 rats or 8 mice) for two separate batches of pooled islets from each of the two isolations.
Human or rodent islet study
- Internal: $2,350
- External: Please request quote
Price includes either an intact islet perfusion study or islet reaggregate dynamic static incubation study for hormone secretion, as well as ½ Insulin ELISA plate for islet study preliminary data and quality assurance.
Additional hormone ELISAs will be charged at cost depending on the type of hormone assay and number of ELISA plates required to run all desired samples. A typical glucose stimulated insulin secretion perifusion study requires 2 ELISA plates per study condition if all critical time points are assayed for first phase and second phase insulin secretion. A typical static glucose stimulated insulin secretion study requires ½ ELISA plate per study condition if a full glucose dose response curve is performed.
Clients are responsible for rodent procurement and housing on their respective IACUC approved animal protocols and can be transferred to the CMC core lab for isolation on the day of the scheduled isolation study.
Human islet procurement can be arranged through the CMC core when donor islets are available. Costs associated with human islet procurement and culture will be charged separately.