We recommend running a mini-gel to verify that your PCR product works.
Determine optimal PCR concentration by serial dilution.
Example of possible microsatellite optimized concentration: pre-PCR = 50 ng/µl, post amplification 25 ng/µl
To avoid Plus A problem, order primer with tails. See User Bulletin: Plus A Artifacts for more information.

Plus A artifacts

To avoid a Plus A problem, order primer with tails. See the User Bulletin: Plus A Artifacts.

User Bulletin: Plus A Artifacts

Specifications

Dye sets and compatibility

Applied Biosystems standard dye sets for genotyping applications
Dye set	DS-30	DS-33
Filter set	D	G5
Dye set components	6-FAM, HEX, NED, ROX	6-FAM, VIC, NED, PET, LIZ
Applied Biosystems kits and other products for genotyping	Custom oligos	AmpFLSTR Identifiler kits, AmpFLSTR Yfiler amplification kits, custom oligos, LMS v2.5, Mouse Mapping Primers, Stockmark kits

Please check the compatibility of your dyes and filter sets with our offerings.

AmpFLSTR 4- and 5-dye kits are not supported on the Applied Biosystems 3730/3730xl instruments.

Sample submission

If including a sample sheet, please email it to DNAseq@Yale.edu.

Sample submission

Rates

The minimum number of samples for a fragment analysis submission is 24 samples. You may submit fewer, but you will still be charged for 24 wells.

If samples are submitted in an incorrect plate type, the user will be charged a re-plate fee.

As of 7/1/2024

Service	Yale user	For-profit user	Non-profit user
Fragment analysis, 96-well plate, per well (Minimum charge is 24 wells)	$1.40	$2.00	$1.75
Re-plate samples, incorrect plasticware	$10.00	$15.00	$15.00

Service

Yale user

For-profit user

Non-profit user

Fragment analysis, 96-well plate, per well

(Minimum charge is 24 wells)

$1.40

$2.00

$1.75

Re-plate samples, incorrect plasticware

$10.00

$15.00

Submission protocols

Yale users: 1-2 µl of each fluorescently labeled PCR product brought to a final volume of 10 µl with water or HiDi formamide.
External users: 1-2 µl of each fluorescently labeled PCR product brought to a final volume of 10 µl with water.

We will add the size standard of your choosing to your submission.

All samples must be submitted in a (non-fully-skirted) 96-well optical plate with clear 2 mL wells. For thicker plastic plates, the angled corner must be in A12. (See the Plasticware specifications page.)

Please fill your plate by column; i.e: A1-H1, A2-H2, and so on.

You may fill out the Sample Sheet Template form if you wish to have specific names for your samples.

It must be sealed properly with strip caps or sealing film to avoid spillage/cross-contamination. If using stop caps, we recommend wrapping the plate with parafilm.

The submission is light sensitive, and should be wrapped in aluminum foil.

If you are shipping your samples, be sure to protect your wells with packing paper, bubble wrap, plate holders, etc. to avoid any breakage.