PCR purification
Keck DNA Sequencing
300 George Street, Floor 2, Room 2127
About the service
PCR purification is a 96-well plate format which is available for researchers who are experienced users of the facility and are confident in their methodology for PCR and primer preparation. We sequence and charge for all wells on the plate regardless of the results, unless it is a clear technical error on our part.
Because the service is plate-based, every well gets processed. Therefore we have to charge for the entire submission, even if there are empty wells.
Standard turnaround time
Standard turnaround time is 1 business day. Yale users will receive priority over non-Yale users. You can check the status of your order on the sample submission page by selecting CHECK EXISTING ORDERS. If you have not received your results in 2 business days, feel free to reach out to us at DNAseq@yale.edu.
If your samples are RUSH samples, please make us aware of this before we receive your submission. We will work to accommodate your request without compromising the lab’s workflow requirements.
Submission requirements
Cap the plate firmly using the strip caps or sealing film and wrap each plate with parafilm. (Sealing film is preferable to strip caps.)
Be sure to clearly label each plate as either PCR plate or Primer plate (or Primer Tube), and label each order with a unique name.
Submissions should be dropped off with an order confirmation sheet (available to print after web ordering via our “Sample Submission” page).
Once we have purified your PCR product, we will then add your primer and sequence your PCR product. Your samples are sequenced by our standard sequencing protocol. If you wish to use another sequencing protocol, please let us know ahead of time.
Results will be posted in your Box account and labeled with the researcher code - plate name -and well-coordinate (RES CODE-plate name-A1-12, RES CODE-plate name-B1-12 etc). The researcher is responsible for holding the "key" to the well co-ordinates for sample identification.
For any questions or concerns, please contact the lab directly, 203-737-2566 or dnaseq@yale.edu.
Available to Yale researchers & external researchers
Core Website
Sample submission
Click herePCR Purification
PLEASE NOTE!! We close for the year at 3PM on Monday, 12/23/2024. Please have your samples in the pickup buckets by 9:30 AM on Monday, December 23, 2024 to ensure processing before the break. You may also bring them in person before 10AM.
Sample SubmissionRates
As of 7/1/2024
| Service | Internal user | External for-profit user | External non-profit user |
|---|---|---|---|
| PCR purification & sequencing, per 96-well plate | $384.00 | $528.00 | $528.00 |
PCR purification plate submission protocol
How to Submit:
PCR product: to be submitted in 96-well plate format with each well containing 20 µl of 15-25 ng/µl of UNPURIFIED PCR product.
Primer(s): to be submitted separately from the PCR products, in 1 of 2 formats: a single tube or a 96-well plate.
- Tube format: Eppendorf tube with 384 µl of 4 µM primer. *ALL products will receive this 1 primer.
- 96-well format: 5 µl of 4 µM primer/well. Primers should be oriented according to PCR plate layout.