QC analysis troubleshooting guide
Questions about your QC results?
This guide is meant to help with some of the most common questions about TapeStation results.
https://www.agilent.com/cs/library/usermanuals/Public/TapeStation_TRB_EN.pdf
Blank Lanes
A blank lane happens when there is very little sample signal, causing any background noise to appear higher than normal. In these cases the sample intensity scale shows very small values.
https://www.agilent.com/cs/library/usermanuals/Public/TapeStation_TRB_EN.pdf
Missing marker peak
CAUSE: Sample too concentrated.
SOLUTION: Dilute your sample until it is in the recommended range.
https://www.agilent.com/cs/library/usermanuals/Public/TapeStation_TRB_EN.pdf
Missing sample peak
CAUSE: Sample too dilute.
SOLUTION: Concentrate your sample until it is in the recommended range.
https://www.agilent.com/cs/library/usermanuals/Public/TapeStation_TRB_EN.pdf
Peak merge with marker
CAUSE: Some sample has run concurrently with the Upper Marker
SOLUTION: Ensure your sample is within the recommended sizing range for the applications.
SOLUTION: Choose the proper assay for your sample size.
Our offerings
Make sure to select the appropriate assay for your samples.
We offer the following assays:
HS DNA 1000
HS DNA 5000
Genomic DNA
HS RNA
Agilent 220 TapeStation System Troubleshooting Manual
For more in depth troubleshooting, you can read Agilent's troubleshooting guide.