Plate sequencing

Plate-based sequencing is a cost-effective and time-saving service recommended for those experienced in their methodology for template and primer preparation.

Minimum charge for this service is 48 samples, but you may submit fewer. We sequence and charge for all samples submitted on the plate, regardless of the results, unless it is a clear technical error on our part. 
 

Sanger sequencing template prep instructions

  •  2uL 4uM Primer + DNA(see table) + (sterile H2O, if necessary) = 18uL
  • Product typeProduct sizeDNA (ng)Ordering notes
    PCR100-300 bp40-50*Can be ordered with Difficult Template or shRNA protocol
    300-500 bp40-160
    500-1000 bp500
    Plasmid  1000-1500
    Single-stranded M13 or phagemic DNA  200-300Order as single-stranded
    BAC and phage lambda DNA 4000Order as BAC 
  • Each reaction should include 2uL of a 4uM Primer. One primer per reaction.
  • The total volume should be 18uL. If extra volume needed, use sterile H2O.
  • Back to Sanger Sequencing
  • Back to Keck DNA Sequencing

Data Return

Standard turnaround time for Sanger services is 1 business day. You can check the status of your order on the sample submission page by selecting CHECK EXISTING ORDERS.

Sample Submission Box.com

Sanger sequencing: plates

Standard turnaround time is 1 business day*. (*CLA turnaround time is 5 business days.) Yale users will receive priority over non-Yale users. If your samples are RUSH samples, please make us aware of this before we receive your submission.

Sample submission Sample sheet template for plate submissions

Submission requirements

Each well of your plate should contain (1) primer and (1) template. 

 

Semi-skirted plates

2 mL semi-skirted plates

Plate specifications

All 96-well plate submissions must be submitted in semi-skirted, 2 mL 96-well plates with clear wells, and sealed with strip caps or sealing film/foil. For more acceptable plate types, please visit our Plasticware requirements page.

Fully skirted, non-transparent wells

Incorrect fully-skirted, non-transparent wells

Incorrect plate type

This plate is an example of an incorrect submission. It is fully skirted. It also has opaque wells. We need the wells to be transparent so we can verify that sample is present, and it helps us to verify the processing is correct.

96-well Plate with strip caps

Plate sealed with strip caps

Labeling and sealing instructions

Be sure to label your submission with your researcher code and your unique plate name.

Cap the plate firmly using the strip caps or sealing film and wrap each plate with parafilm. (Sealing film is preferable to strip caps.)

Plate filled by rows

Plate correctly filled A01-A12, B01-B12, etc.

Correct plate setup

When submitting fewer than 96 samples on a plate, please submit the samples in consecutive rows. Begin filling the wells at A1 through A12, then B1-B12, C1-12, etc.. If there are any empty wells in the middle of your submission, you will be charged for them.

Incorrect plate setup

Incorrect plate filling scheme

Incorrect plate setup

This plate is filled incorrectly; it has missing wells and does not run from well 1-12 in a row.

Confirmation Page

Order confirmation sheet

Confirmation sheet

Plate submissions should be dropped off with an order confirmation sheet (available to print after web ordering via our “Sample submission” page).

Sample sheet template

Optional sample sheet format

[Optional] sample sheet

You may submit a sample sheet with your submission if you wish. If you do not send us an Excel form with your sample names, they will be named as follows: [Researcher code]-[Plate name]-[well position].

PLEASE NOTE!! Our facility will be closed on Thursday, November 28 & Friday, November 29, 2024 in observance of the University Holiday. Please have your samples in the pickup buckets by 9:30 on Wednesday, November 27 to ensure processing before the break.

NSF CAREER Training Series – Join Us!

The series is designed to support early-career faculty in preparing competitive proposals for the NSF Faculty Early Career Development (CAREER) Program.