Plate sequencing

Plate-based sequencing is a cost-effective and time-saving service recommended for those experienced in their methodology for template and primer preparation.

Minimum charge for this service is 48 samples, but you may submit fewer. We sequence and charge for all samples submitted on the plate, regardless of the results, unless it is a clear technical error on our part.

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Sanger sequencing template prep instructions

All samples should include 2 uL of a 4 uM primer.
Total volume should be 18 uL. If extra volume is needed, use sterile H₂O.

Product size	Product type	DNA (ng)	Ordering notes
100-300 bp	PCR	40-50	*Can be ordered with Difficult Template or shRNA protocol
300-500 bp		40-160
500-1000 bp		500
	Double-stranded plasmid DNA	1000-1500
	Single-stranded M13 or phagemic DNA	200-300	Order as single-stranded
	BAC and phage lambda DNA	4000	Order as BAC

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Data Return

Standard turnaround time for Sanger services is 1 business day. You can check the status of your order on the sample submission page by selecting CHECK EXISTING ORDERS.

Sample Submission Box.com

Submission requirements

Each well of your plate should contain (1) primer and (1) template.

Semi-skirted plates — 2 mL semi-skirted plates

Plate specifications

All 96-well plate submissions must be submitted in semi-skirted, 2 mL 96-well plates with clear wells, and sealed with strip caps or sealing film/foil. For more acceptable plate types, please visit our Plasticware requirements page.

96-well Plate with strip caps — Plate sealed with strip caps

Labeling and sealing instructions

Be sure to label your submission with your researcher code and your unique plate name.

Cap the plate firmly using the strip caps or sealing film and wrap each plate with parafilm. (Sealing film is preferable to strip caps.)

Plate filled by rows — Plate correctly filled A01-A12, B01-B12, etc.

Correct plate setup

When submitting fewer than 96 samples on a plate, please submit the samples in consecutive rows. Begin filling the wells at A1 through A12, then B1-B12, C1-12, etc.. If there are any empty wells in the middle of your submission, you will be charged for them.

Incorrect plate setup

This plate is filled incorrectly; it has missing wells and does not run from well 1-12 in a row.

Confirmation sheet

Plate submissions should be dropped off with an order confirmation sheet (available to print after web ordering via our “Sample submission” page).

Sample sheet template — Optional sample sheet format

[Optional] sample sheet

You may submit a sample sheet with your submission if you wish. If you do not send us an Excel form with your sample names, they will be named as follows: [Researcher code]-[Plate name]-[well position].