Flow cytometry analysis
About the instruments
The Yale Flow Cytometry Core is equipped with user-operated analyzers at three different locations: The Anlyan Center (TAC) on the 5th and 6th floors, Amistad, and 300 George Street. The TAC facility houses the LSRII TAC6-GS, two CytoFlex LX cytometers, a BD Symphony as well as a spectral Cytek Aurora cytometer. Additionally, there is a 5-laser LSR Fortessa located in Amistad. At the 300 George Street location, a Cytek Aurora, Symphony B and another 5-laser LSR Fortessa are available. Flow cytometry analysis starts with scheduling and completing a required training session. All training requests can be made in PPMS. Please review our Onboarding page to get familiar with the process and schedule a training.
We are open to all researchers throughout the Yale campus. New users should contact Diane Trotta.
Cytometers
Instrument | Location | Contact email | Contact phone |
---|---|---|---|
Amnis Imagestream | TAC S613 | Ashraf Khalil | 203-785-7958 |
Aurora | TAC S617 | Jennifer Kelly | 203-737-7452 |
BD LSR Fortessa | Amistad Room 105 | Ewa Menet | 203-785-2299 |
BD LSRII Amistad (Ami) | TAC S533 | Diane Trotta | 203-785-7958 |
BD LSRII TAC6-GS | TAC S617 | Diane Trotta | 203-785-7958 |
CytoFlex LX | TAC S613 | Diane Trotta | 203-785-7958 |
BD Symphony A | TAC S613 | Diane Trotta | 203-785-7958 |
BD Symphony B | 300 George St Lab 2331 | Jennifer Kelly | 203-737-7452 |
BD LSR Fortessa | 300 George St Lab 2331 | Lesley Devine | 203-785-2031 |
Luminex | 300 George St Lab 2331 | Lesley Devine | 203-785-2031 |
Available to Yale researchers & external researchers
Downloadable files
Yale Flow Core Instrumentation at a GlanceRates
Description | Fee |
---|---|
Mid-tier (CytoFlex LX, LSRII, Fortessa, Amnis) | $48/hr |
Hi-tier (Symphony) | $52/hr |
Mid-tier analyzer training | $37/hr session |
Staff operation of analyzer and/or data analysis (staff effort only; instrument charge may apply) | $75/hr |
Luminex (independent operator) | $70/plate |
FlowJo site license seat ($20 - $30 dependent on subscriber #) | currently $18.08/seat/month |
Training & services
Do you need flow cytometry analysis, cell sorting, or both? Analysis results in data recorded by acquisition software. Cell sorting separates cells into pure populations and all the data acquired during sorting.
All scheduling, training requests, and services are made through an online portal, PPMS. To log onto the PPMS, point your browser to https://ppms.us/yale/start/. Your Yale NetID and password will serve as your account credentials. (Download a short guide to this intuitive login.) Please confirm your lab affiliation (“group”) and charging instructions. We also recommend that you check that your permissions for instrument access are correct. In most cases, details about your account may pre-populate, but new users and those without affiliated COAs will need to wait for the business office to approve the COAs you list before your account is functional. There might therefore be a short delay after your account creation before you can book equipment with this system. Once your account is functional, you will be able to book instruments and review reservations with a much more sophisticated interface.
More information
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trainingrequestguide-flowcytometry.pdf (2.42 MB)