Analysis: LSRII GS
Location & contact
The Anlyan Center (TAC) 6
Diane Trotta, 203-785-7959
Tables
Violet laser: 405 nm 100 mW
| PMT | Bandpass | Long pass | Fluorochrome |
|---|---|---|---|
| A | 710/50 | 690 | BV711 |
| B | 610/20 | 595 | BV605, Qdot 605 |
| C | 525/50 | 505 | AmCyan, CFP |
| D | 450/50 | Pacific blue, Alexa Fluor 405, CFP, BFP, Cascade blue, Sky blue, BV421 |
Blue laser: 488 nm 50 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
|---|---|---|---|
| A | 685/35 | 670 | PerCP |
| B | 525/50 | 505 | FITC, GFP, Alexa 488, CFSE, YFP |
| C | 488/10 | SSC |
Green laser: 532 nm 150 mW
| PMT | Bandpass | Long pass | Fluorochrome |
|---|---|---|---|
| A | 780/60 | 750 | PE-Cy7 |
| B | 710/50 | 685 | PE-Cy5.5 |
| C | 670/30 | 635 | PE-Cy5 |
| D | 610/20 | 600 | PE-Texas Red, PI, AlexaFluor 594, AlexaFluor 568, Rhodamine-B |
| E | 582/15 | PE, DS Red, RFP, Alexa Fluor 532 |
Red laser: 640 nm 70 mW
| PMT | Bandpass | Long pass | Fluorochrome |
|---|---|---|---|
| A | 780/60 | 750 | APC-Cy7, Alexa Fluor 750 |
| B | 710/25 | 685 | Alexa Fluor 700, Alexa Fluor 680 |
| C | 670/30 | APC, Alexa Fluor 647 |