UV laser: 355 nm 60 mW
| PMT | Bandpass | Long pass | Fluorochrome |
|---|
| A | 740/35 | 690LP | BUV737 |
| B | 450/50
| 410LP | DAPI**, Alexa Fluor 350
|
| C | 379/28 | | BUV395** |
**DAPI and BUV395 should not be used together. Additional filters are necessary for calcium flux and side population detection. Please see the staff about filter swaps.
Violet laser: 405nm 50 mW
| PMT | Bandpass | Long pass | Fluorochrome |
|---|
| A | 710/50 | 690LP | BV711 |
| B | 610/20 | 595LP | BV605, Alexa Fluor 605, QDot605 |
| C | 525/50 | 505LP | Alexa Fluor 430, CFP, Am Cyan, Pacific Orange |
| D | 450/50 | | Cascade Blue, Pacific Blue, Sky Blue, Alexa Fluor 405, BFP, CFP, Hoechst
|
Blue laser: 488 nm 50 mW
| PMT | Bandpass | Long pass | Fluorochrome |
|---|
| A | 685/35 | 630LP | PerCP, EMA, PercP-Cy5.5 |
| B | 525/50 | 505LP | FITC, GFP, PKH67, CFSE, Alexa Fluor 488, YFP* |
| C | 488/10 | | SSC |
*YFP: If detecting YFP and GFP, ask for filter changes on A and B PMTs.
Green laser: 532 nm 150 mW
| PMT | Bandpass | Long pass | Fluorochrome |
|---|
| A | 780/60 | 735LP | PE-Cy7, PE-Alexa750 |
| B | 710/50 | 685Lp | PE-Cy5.5 |
| C | 675/20 | 630LP | PE-Cy5, PE-Alexa647 |
| D | 610/20 | 600LP | Alexa Fluor 594*, PE-Texas Red, Alexa Fluor 568**, PI, Rhodamine-B, PI |
| E | 575/26 | | PE, DsRed, RFP, Pyronin-Y, PKH26 |
* Alexa Fluor 594 only 25% Ex.
**Alexa Fluor 568 only 34% Ex.
Red laser: 640 nm 70 mW
| PMT | Bandpass | Long pass | Fluorochrome |
|---|
| A | 780/60 | 755LP | APC-Cy7, Alexa Fluor 750* |
| B | 710/50 | 690LP | Alexa Fluor 680, Alexa Fluor 700 |
| C | 660/20 | | APC, Alexa Fluor 647 |
*Alexa Fluor 750 only 18% Ex.