Analysis: CytoFlex LX A and B
Location — Training — Specifications — Table
Both CytoFLEX analyzers are a 5-laser system capable of detecting in 19 different channels (please see the configuration below for details), with a slight difference: CytoFLEX A has a VSSC detector off the violet laser to allow for small particle detection, while CytoFLEX B has a BV786 detector. Otherwise, both instruments have the same configuration.
Location
• Cytoflex A - TAC (The Anlyan Center) S613
• Cytoflex B - TAC S613
Training
For training, please contact Diane Trotta.
Specifications
The CytoFLEXes are equipped with a 561 green laser. This is different from the Stratedigms, which they have replaced and our LSRIIs with 532 green lasers.
- You should expect your PE and PE-tandem dyes to appear brighter than they did before. Less antibody might be required to avoid extreme adjustments on the sensitive detectors of this model.
- Some fluorescent proteins that were barely detectable with a 532 nm laser will appear significantly brighter with 561 nm excitation. mCherry, mKate, mKatushka, and mRaspberry will perform much better with this laser line.
CytoflexLX A and B have almost the same configurations. Only one detector difference off violet laser. VSSC vs. BV786.
| Laser (NM) | Bandpass | Channel Name | Additional Optimal Colors |
|---|---|---|---|
| 355 | 405/30 | BUV395 | |
| 450/45 | DAPI | ZombieUV, L/DFix Blue, DyLight 350 | |
| 740/35 | BUV737 | ||
| 405 | 405/10 | VSSC (CytoA only) | Violet Side Scatter - used for small particle detection |
| 450/45 | PacificBlue | BV421, V450, Alexa405, CF405M, DyLight405, eFluor450, SuperBright436, VioBlue, Zombie Violet, L/DFixViolet | |
| 525/40 | BV510 | L/DFix Aqua, V500, AF430, DyLight405LS, VioGreen, Zombie Aqua | |
| 610/20 | BV605 | Qdot605, DyLight405LS, eFluor605NC, eVolve605, SuperBright600 | |
| 712/25 | BV711 | Qdot705, SuperBright702 | |
| 763/43 | BV786 (CytoB only) | Qdot800 | |
| 488 | 525/40 | FITC | GFP, Alexa488, CFSE, BB515, DyLight488, Zombie Green, L/Dfix Green |
| 610/20 | B610-ECD | all current available fluors for this channel are best excited by the 561 laser | |
| 690/50 | PerCP | PerCP-Cy5.5, BB700, PerCP-eFluor710, PerCP-Vio700 | |
| 561 | 584/42 | PE | dsRed, RFP |
| 610/20 | PE-TexasRed | mCherry, PE-Dazzle, PE-CF594, PE-Vio615, PE-eFluor610, DyLight594, Zombie Red, L/Dfix Red | |
| 675/30 | PE-Cy5 | mPlum | |
| 710/50 | PE-Cy5.5 | ||
| 763/43 | PE-Cy7 | PE-Vio770 | |
| 638 | 660/10 | APC | AF647, Dylight633, L/Dfix Far Red |
| 712/25 | AL680 | Alexa700 | |
| 763/43 | APC-Cy7 | APC-Fire750, APC-Alexa750, APC-eFluor780, APC-Vio770, Zombie NIR, L/Dfix NIR |
* L/Dfix = Live/dead fixable