Invitrogen Attune NXT Cell Analyzer
Available to Yale researchers & external researchers
Specifications
| Attune Fluorophore Specifications | |||
| Channel | Filter | ||
| Violet - 405 nm | |||
| VL1 | 440/50 | AF405. L/D Fixable Violet, DAP, Pac Blue, Celltrace Violet, BV421, eFluor 450, Horizon V450, eBFP, eCFP, mTourquoise | |
| VL2 | 512/25 | PacGreen, L/D Fixable Aqua, BV 510, AmCyan, Horizon V500, VioGreen, vGFP | |
| VL3 | 603/48 | PacOrange, L/Dfixable Yellow, BV570, BV605, BV650, eFluor 605NC | |
| VL4 | 710/50 | Qdot 705, BV650, BV 711 | |
| Blue - 488 nm | |||
| BL1 | 530/30 | FITC, AF 488, BB515, CFSE, eGFP, eYFP | |
| BL1 | 574/26 | BB515, AF 488, GFP, CFSE, Yo Pro1, Calcein, VioBright 515, Spark Blue 574, eFluor 520 | |
| Yellow - 561 nm | |||
| YL1 | 585/16 | PE, PI, AF568, AF594, dTomato, mOrange, mApple, RFP | |
| YL2 | 620/15 | PE-AF610, PE-TxRed, dsRed, mCherry, mKate, mStrawberry, PE-CF594, PE/Dazzle594, PE-eFluor 610, PE-Vio 610 | |
| YL3 | 695/40 | PE-AF700, PE-Cy5.5, 7-AAD | |
| YL4 | 780/60 | PE-Cy7, PE-Vio770 | |
| Red - 637 nm | |||
| RL1 | 670/14 | AF647, APC, LD/Far Red, AF680, eFluor 660 | |
| RL2 | 720/30 | AF680, AF700, APC-AF700, | |
| RL3 | 780/60 | APC-Cy7, APC-AF750, L/DNearIR, APC-H7, APC-eFluor750, APCeFluor-780, APC-Vio770 | |
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Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration are assessed with the Agilent Seahorse XF Pro. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. An integrated Agilent BioTek Cytation5 imager correlates cell number to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS
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Instrument/Equipment
BD Symphony S6 Cell Sorter
Sort into 5 mL round tubes, 15 mL conical tubes, or plates (24-, 48-, and 96-well) with 5 lasers (UV, violet, blue, yellow/green, red) and 23 detectors (6 UV, 6 violet, 4 blue, 4 yellow/green, 3 red).
Located within West Campus Flow Cytometry Facility -
Instrument/Equipment
Flow cytometry cell sorting
Learn more about cell sorting at Yale Flow Cytometry Facility.
Located within Yale Flow Cytometry Facility