Invitrogen Attune NXT Cell Analyzer
Analyze cells from tubes and plates with 4 lasers (violet, blue, yellow/green, red) and 14 detectors (6 violet, 2 blue, 3 yellow, 3 red).
Available to Yale researchers & external researchers
Specifications
| Attune Fluorophore Specifications | |||
| Channel | Filter | ||
| Violet - 405 nm | |||
| VL1 | 440/50 | AF405. L/D Fixable Violet, DAP, Pac Blue, Celltrace Violet, BV421, eFluor 450, Horizon V450, eBFP, eCFP, mTourquoise | |
| VL2 | 512/25 | PacGreen, L/D Fixable Aqua, BV 510, AmCyan, Horizon V500, VioGreen, vGFP | |
| VL3 | 603/48 | PacOrange, L/Dfixable Yellow, BV570, BV605, BV650, eFluor 605NC | |
| VL4 | 710/50 | Qdot 705, BV650, BV 711 | |
| Blue - 488 nm | |||
| BL1 | 530/30 | FITC, AF 488, BB515, CFSE, eGFP, eYFP | |
| BL1 | 574/26 | BB515, AF 488, GFP, CFSE, Yo Pro1, Calcein, VioBright 515, Spark Blue 574, eFluor 520 | |
| Yellow - 561 nm | |||
| YL1 | 585/16 | PE, PI, AF568, AF594, dTomato, mOrange, mApple, RFP | |
| YL2 | 620/15 | PE-AF610, PE-TxRed, dsRed, mCherry, mKate, mStrawberry, PE-CF594, PE/Dazzle594, PE-eFluor 610, PE-Vio 610 | |
| YL3 | 695/40 | PE-AF700, PE-Cy5.5, 7-AAD | |
| YL4 | 780/60 | PE-Cy7, PE-Vio770 | |
| Red - 637 nm | |||
| RL1 | 670/14 | AF647, APC, LD/Far Red, AF680, eFluor 660 | |
| RL2 | 720/30 | AF680, AF700, APC-AF700, | |
| RL3 | 780/60 | APC-Cy7, APC-AF750, L/DNearIR, APC-H7, APC-eFluor750, APCeFluor-780, APC-Vio770 | |
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Services
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Instrument/Equipment
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Located within West Campus Flow Cytometry Facility