Listing of research core items
-
Instrument/equipment
Electron-beam lithography
For nanoscale patterning, the Raith EBPG 5000+ and EBPG 5200+ electron-beam lithography systems provide 100kV patterning of 10 nm scale devices.
-
Instrument/equipment
-
Services
Metabolic flux and enrichment studies using MIMOSA
In this subcore, parameters of cellular and tissue metabolic flux are assessed by incorporation of stable isotope labeled tracers (e.g., 13C, 15N, 2D) into metabolism. The CMC uses the targeted mass isotopomer multi-ordinate spectral analysis (MIMOSA) platform to interpret stable isotope labeling patterns for calculation of rates of discrete steps in glycolytic and mitochondrial metabolism. Such measurements can be used to identify differences in fuel usage (e.g., glycolysis vs. beta-oxidation), and non-oxidative (reductive carboxylation, anaplerosis and cataplerosis) contributions to the TCA cycle.
Located within Chemical Metabolism CoreStephan Siebel, MD, PhD
-
Instrument/equipment
NMR
Our NMR instruments include a Bruker 400 MHz broadband probe and an Agilent DD2 600 MHz 3 mm cold probe.
Located within West Campus Analytical Core -
Instrument/equipment
Transmission electron microscopy
The FEI Tecnai Osiris 200kV TEM is specifically configured for material science.
Sungwoo Sohn, PhD
-
Instrument/equipment
-
Instrument/equipment
Scanning electron microscopy
Two Hitachi SU-70 scanning electron microscopes provide 1 nm resolution with a high-brightness electron source.
-
Instrument/equipment
3D printing
At the APC we have three 3D printers: a Formlabs Form 3, a Dremel 3D20, and a Lulzbot Taz Pro.
Located within Advanced Prototyping Center at Wright LabJames A. Nikkel, PhD
-
Instrument/equipment
Atomic force microscopy
A Bruker Dimension Fastscan AFM provides atomic force microscopy in both air and fluids using the new “Peak Force” tapping mode as well as very high speed resonant tapping mode.
-
Services
Islet isolation and function
In this subcore, our technical expertise in isolating rodent islets including rats, mice and most recently ground squirrel islets, from the pancreas ex-vivo allows researchers to study the intact islets for their studies or to proceed with substrate stimulated insulin secretion functional studies using our BioRep Technologies Islet Perfusion Systems (8-channel and 12-channel) offered by our core. For non-rodent islet studies, human donor islet procurement assistance as well as unction studies are also offered.
Located within Chemical Metabolism CoreRebecca Cardone, MS
-
Instrument/equipment
-
Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration are assessed with the Agilent Seahorse XF Pro. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. An integrated Agilent BioTek Cytation5 imager correlates cell number to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS