Transmission electron microscopy
Sungwoo Sohn, PhD
About the instrument
While the software and electron optics share a great deal with the 200kV TEMs found in the Yale Medical School, the FEI Tecnai Osiris 200kV TEM is equipped with a new type of high-brightness electron source and a new high-area solid-angle x-ray detector, making possible high-speed spectral imaging. This new generation of electron microscope is operated from a remote console, making use of motorized apertures and high-resolution video cameras to keep human operators removed from the sensitive electron optics.
Available to Yale researchers & external researchers
Specifications
| Point resolution | 0.25 nm |
| Information limit | 0.14 nm |
| Spherical aber. coef. | 1.2 mm |
| Chromatic aber. coef. | 1.1 mm |
| Maximum tilt | ±30 |
| Brightness @200kV | 1.8e9 A/cm²srad |
| EDX resolution | 136 eV |
Rates
Rates in US$/hour unless otherwise noted
| Yale academic | External academic | Industrial | |
|---|---|---|---|
| SEM, Hitachi | 37 | 61 | 215 |
| AFM, Bruker | 35 | 58 | 105 |
| e-beam, EBPG | 100 | 165 | 300 |
| TEM, FEI Osiris | 100 | 165 | 300 |
| Ion mill, Fischione | 37 | 61 | 111 |
| Ion mill, Hitachi | 37 | 61 | 111 |
| Gold sputtering | 20 | 32 | 76 |
| Iridium sputtering | 20 | 32 | 76 |
| Microtome, Leica | 30 | 50 | 90 |
| HSQ resist, 4 ml | 25 ea | 41 ea | 75 ea |
| CSAR resist, 4 ml | 20 ea | 33 ea | 60 ea |
| Dektak profilometer | 10 | 16 | 45 |
Training & services
We have recorded a set of video clips for TEM training. You will be at the TEM, watching and copying the videos, while the instuctor is nearby and available for questions. You will stop the videos at various times so that the instructor can check your progress. This seems to work pretty well, and people like going through the course at their own pace. You might take one hour, or you might take four hours. It’s up to you. Sign up for TEM training by using this appointment calendar.
The hard part of TEM training is showing you how to use the load lock. But we found a way! The Gibbs shop made us a “load lock simulator” that lets you practice without abusing the vacuum pumps. Neat!
Contact
Cheat sheets
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tem_cheat_sheet_v6.pdf (669.38 KB)TEM cheat sheet
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flat_polishing.pdf (653.29 KB)Flat polishing
TEM prep tools
In support of TEM sample preparation, the YINQE laboratory is also equipped with a Fischione 1050 ion-beam thinning tool, a focused ion-beam instrument, and a cryo-microtome.
Microtome
A microtome makes possible the sectioning of soft material for TEM analysis. Accessories for the Leica Ultra UC7 include a trimming mill and a glass knife maker.
Materials suitable for microtome slicing include polymer beads, carbon nanotubes, electro-spun fibers, and soft metals. YINQE supports only room-temperature operation.
More expertise in low-temperature sectioning and staining can be found at the Yale Medical School TEM facility.