Listing of research core items
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Instrument/equipment
Electron-beam lithography
For nanoscale patterning, the Raith EBPG 5000+ and EBPG 5200+ electron-beam lithography systems provide 100kV patterning of 10 nm scale devices.
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Services
Global metabolomics and integrative -omics (intOmics)
Metabolomics provides screening of targeted (~650) and untargeted (~2500) metabolites. Options for more detailed analyses include volcano plots, pathway enrichment and KEGG network optimization data. Metabolomic data can be integrated with (user provided) RNAseq using a metabolomics network analysis platform (IntOmix), for an optimized pathway analysis of relationships between metabolites and enzyme transcripts.
Located within Chemical Metabolism CoreStephan Siebel, MD, PhD
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Instrument/equipment
Transmission electron microscopy
The FEI Tecnai Osiris 200kV TEM is specifically configured for material science.
Sungwoo Sohn, PhD
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Services
Quantitative targeted analysis
Identification and quantification of key metabolites of central carbon metabolism.
Located within Chemical Metabolism CoreStephan Siebel, MD, PhD
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Instrument/equipment
Scanning electron microscopy
Two Hitachi SU-70 scanning electron microscopes provide 1 nm resolution with a high-brightness electron source.
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Instrument/equipment
Atomic force microscopy
A Bruker Dimension Fastscan AFM provides atomic force microscopy in both air and fluids using the new “Peak Force” tapping mode as well as very high speed resonant tapping mode.
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Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration are assessed with the Agilent Seahorse XF Pro. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. An integrated Agilent BioTek Cytation5 imager correlates cell number to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS
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Instrument/equipment
Bruker Luxendo light sheet microscope
Bruker Luxendo light sheet microscope
Located within Confocal Microscopy at CCMIMateus Guerra, PhD
Primary contact Associate Director -
Instrument/equipment
Bruker Opterra II swept-field microscope
Bruker Opterra II swept-field microscope
Located within Confocal Microscopy at CCMIMateus Guerra, PhD
Primary contact Associate Director -
Instrument/equipment
Bruker Vutara 352 super-resolution microscope
Bruker Vutara 352 super-resolution microscope
Located within Confocal Microscopy at CCMIMateus Guerra, PhD
Primary contact Associate Director -
Core/facility
Yale Center for Genome Analysis (YCGA)
The Yale Center for Genome Analysis is a full-service facility dedicated to providing RNA expression profiling, DNA genotyping, and high-throughput sequencing using state-of-the-art technologies.
Primary contact
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Core/facility
Yale Center for Molecular Discovery
We provide resources for assay development and high-throughput screening with small molecules and arrayed siRNA and sgRNA/CRISPR libraries in biochemical, cell-based and image-based assays (primarily 384-well format).