Spatial genomics and proteomics analysis
About the services
10x Genomics Visium gene expression
With Protein co-detection, it is a next-generation molecular profiling solution for classifying tissue based on total mRNA. Map the whole transcriptome with morphological context in FFPE or fresh frozen tissues to discover novel insights into normal development, disease pathology, and clinical translational research.
NanoString GeoMx DSP
The GeoMx Digital Spatial Profiler (DSP) provides morphological context in spatial transcriptomics and spatial proteomics experiments from just one slide.From discovery to translational research, the GeoMx DSP is the most flexible and robust spatial biology solution designed to conform to your ever-changing research needs.
The GeoMx DSP workflow seamlessly integrates with current histology or genomics workflows to help researchers obtain robust and reproducible spatial multiomics data quickly.
Whole tissue sections, FFPE or fresh frozen, can be imaged and stained for RNA or protein. Researchers can then precisely select which tissue compartments or cell types to profile based on the biology, and subsequently count expression levels using either the nCounter Analysis System or an Illumina Sequencer.
Available to Yale researchers & external researchers
Core websiteSpecifications
Sample submission
Please contact Guilin Wang for 10x Visium or Nanostring GeoMX service request.
Rates
As of 7/1/23
| Service | Yale user | Non-Yale user |
| 10x Visium library prep (per section, minimal 4 sections) | $1876 | $2440 |
| 10x Visium CytAssist GEX (per section, minimal of 2rxn\1 slide 11mm) | $4028 | $5260 |
| 10x Visium CytAssist GEX (per section, minimal of 2rxn\1 slide 6.5mm) | $2100 | $2767 |
| 10x Visium CytAssist GEX (without reagents) | $344 | $475 |
| NanoString DSP library prep | $553 | $748 |
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Services
Global metabolomics and integrative -omics (intOmics)
Metabolomics provides screening of targeted (~650) and untargeted (~2500) metabolites.
Located within Chemical Metabolism CoreStephan Siebel, MD, PhD
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Services
Quantitative targeted analysis
Identification and quantification of key metabolites of central carbon metabolism.
Located within Chemical Metabolism CoreStephan Siebel, MD, PhD
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Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration (OCR) and acidification (ECAR) are assessed with the Agilent Seahorse XF Pro 96. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. Changes in acidification are also observed simultaneously. An integrated Agilent BioTek Cytation5 imager correlates cell number via Hoechst nuclear stain to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS