Listing of research core items
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Services
Global metabolomics and integrative -omics (intOmics)
Metabolomics provides screening of targeted (~650) and untargeted (~2500) metabolites.
Located within Chemical Metabolism CoreStephan Siebel, MD, PhD
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Services
Quantitative targeted analysis
Identification and quantification of key metabolites of central carbon metabolism.
Located within Chemical Metabolism CoreStephan Siebel, MD, PhD
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Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration (OCR) and acidification (ECAR) are assessed with the Agilent Seahorse XF Pro 96. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. Changes in acidification are also observed simultaneously. An integrated Agilent BioTek Cytation5 imager correlates cell number via Hoechst nuclear stain to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS
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Core/facility
Keck Bioinformatics Resource
The Bioinformatics Resource offers software support; consultation on DNA/protein sequence, microarray, protein structure, and pathway analysis; and collaboration.
Contact
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Core/facility
Keck Biostatistics Resource
We offer consultation related to statistical and computational analysis of various types of biological and biomedical data, such as gene expression, genotyping, mass spectrometry, and next-generation sequencing.
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Core/facility
Yale Center for Genome Analysis (YCGA)
The Yale Center for Genome Analysis is a full-service facility dedicated to providing RNA expression profiling, DNA genotyping, and high-throughput sequencing using state-of-the-art technologies.
Primary contact
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Core/facility
Yale Center for Molecular Discovery
We provide resources for assay development and high-throughput screening with small molecules and arrayed siRNA and sgRNA/CRISPR libraries in biochemical, cell-based and image-based assays (primarily 384-well format).
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Core/facility
Chemical Metabolism Core
Previously called the Islet, Oxygen consumption, Mass Isotopomer flux Core (IOMIC), the Chemical Metabolism Core offers targeted and untargeted metabolomics, stable isotope-based metabolic flux analysis, quantitative metabolic panels of central carbon metabolism and adjacent pathways, measurements of cellular bioenergetics, islet isolation and functional studies. Services include study assistance and data analysis.
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Core/facility
CyTOF Facility
The CyTOF 2 facility houses a time-of-flight atomic mass spectrometer for the high-speed acquisition of highly multi-parametric single-cell data.
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Core/facility
Yale University Cytogenetics Laboratory
The core provides testing for cancer predisposition, metabolic and developmental disorders.
DNA Diagnostics Lab
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Core/facility
Keck DNA Sequencing Facility
Keck DNA Sequencing provides Sanger sequencing and other services, with costs starting as low as $1.99/well and $3.50/tube submission. Sequencing is performed using Thermo Fisher technologies.
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Services
DNA/RNA QC analysis service
QC using the Agilent Tapestation 2200 allows researchers to analyze the quantity and size of DNA or RNA while using only a few microliters of precious sample.
Located within Keck DNA Sequencing FacilityKeck DNA Sequencing