Leica SP8 Gated STED 3X super resolution

The Leica SP8 Gated STED 3X super-resolution microscope is based on the STED (STimulated Emission Depletion) principle. It provides fast, intuitive, and purely optical access to study subcellular architecture and dynamics at the nanoscale. Resolution is approximately 50 to 60 nm, which is a 5-fold increase compared to diffraction-limited confocal systems. 

The microscope is equipped with a 405 nm laser and a tunable (460 nm-660 nm) pulsed white-light laser for excitation. Two PMT detectors and two HyD detectors are available for tunable spectral detection. Three STED depletion lasers (592 nm, 660 nm, and 775 nm) make 3-dye super-resolution possible. For live-cell imaging, a temperature and CO2 controlled chamber is available, along with a resonate scanner which is capable of scan speeds 10x faster than traditional galvanometric scanners.

The Leica LAS-X software interacts seamlessly with Huygens STED deconvolution software for image enhancement through Leica’s integrated hyvolution interface.