Instrument/Equipment Core/Facility: Confocal Microscopy at CCMI

Leica SP8 Gated STED 3X Super Resolution

Leica SP8 Gated STED 3X Super Resolution microscope

Available to Yale researchers & external researchers

Core Website

Michael H. Nathanson, MD, PhD

Request Services or Equipment Access
Hours & Location

Sterling Hall of Medicine Building, room IE-25

About the Leica SP8 Gated STED 3X Super Resolution

The Leica SP8 Gated STED 3X super-resolution microscope is based on the STED (STimulated Emission Depletion) principle. It provides fast, intuitive, and purely optical access to study subcellular architecture and dynamics at the nanoscale. Resolution can be increased 5-fold compared to diffraction-limited confocal systems.

The microscope is equipped with a 405 nm laser and a tunable (460 nm-660 nm) pulsed white-light laser for excitation. Two PMT detectors and two HyD detectors are available for tunable spectral detection. Three STED depletion lasers (592 nm, 660 nm, and 775 nm) make 3-dye super-resolution possible. For live-cell imaging, a temperature and CO2 controlled chamber is available, along with a resonate scanner which is capable of scan speeds 10x faster than traditional galvanometric scanners.

The Leica LAS-X software interacts seamlessly with Huygens STED deconvolution software for image enhancement through Leica’s integrated hyvolution interface.


ServiceYale FacultyNon-Yale Users
Microscopes$60/hr$120/hr for for-profit; $75/hr for non-profit
Workstations$5/hr$7/hr for both for-profit and non-profit