Bruker Opterra II swept-field microscope
Bruker Opterra II swept-field microscope
Available to Yale researchers & external researchers
Sterling Hall of Medicine, room IE-25
About the Bruker Opterra II swept-field microscope
The Bruker Opterra II swept-field microscope can collect images as quickly as 500 fps and is particularly useful for live-cell imaging. The microscope uses one-dimensional pinhole arrays or slits for increased acquisition rate while maintaining confocal resolution and elimination of crosstalk, a common problem associated with spinning-disk confocals. A pair of EMCCD cameras enable 2-color simultaneous imaging, allowing the fast acquisition rate. The camera's sensitivity ensures minimized photobleaching and phototoxicity in live cells and embryos.
The system is equipped with a live-cell incubation chamber for control of temperature, humidity and CO2. Additionally, the microscope has a photo activation module with 405 nm and 488 nm lasers.
Rates
Service | Yale Faculty | Non-Yale Users |
---|---|---|
Microscopes | $60/hr | $120/hr for for-profit; $75/hr for non-profit |
Training | Free | Free |
Workstations | $5/hr | $7/hr for both for-profit and non-profit |