Instrument/equipment Core/facility: Light Microscope Imaging Facility on Science Hill

Zeiss Lightsheet Z.1 Microscope

Joseph Wolenski, PhD

Building:

Yale Science Building, room C181A

About the instrument

The Zeiss Lightsheet Z.1 dual illumination microscope is a selective plane illumination microscope (SPIM) that excites the sample with a plane of laser light from dual ports for ultrafast 4D sections of live transparent organisms, including: zebrafish embryos, Drosophila, and cleared organs.

Available to Yale researchers only

Core website

Specifications

Laser lines:
     405 nm, 50 mW
     445 nm, 25 mW
     488 nm, 50 mW
     515 nm, 20 mW
     561 nm, 50 mW
     638 nm, 75 mW

ZEN 2 software with the following modules:
     Arivis vision 4D
     Measurement

Image analysis

Lightsheet Z1 package for 3D Multiview

Lightsheet detection module: up to 30 fps at 960 x 1024 pixels.

Objectives (water immersion).

     5x/0.16 NA
     10x/0.5 NA
     20x/1.0 NA
     40x/1.0 NA

Rates

Rate for FY 24: $52/hour

Monthly billing is not capped as of August 1, 2021.