Services

Fee-for-service workflows

Our fee-for-service workflows include:​

• Protein ID from the following:
  • Gel bands
  • IP/co-IP proteins
  • Cells: FACs sorted/enriched cell-type/subcellular/pellet/lysate
  • Tissue: whole/specific regions (PFC, DS, cerebellum, etc. of brain) / laser capture microdissection / proximity labeling
• Protein posttranslational modification (PTM) identification
• Protein profiling and quantitation. Approaches:
  • Label-free quantitation (DDA/DIA)
  • TMT multi-plex
  • SILAC
  • Labeled
• Intact protein determination (<40 kDa)
• High-resolution (HR) MS for small molecule and structural elucidation​
• Targeted quantitation of small molecule​s
• Targeted proteomics (parallel reaction monitoring [PRM])

Services in detail

Protein/peptide fractionation

• Reverse-phase HPLC (high or low pH)
• Strong cation exchange HPLC

Post-translational modifications (PTMs)

• Phosphorylation & phosphoproteomics
  • Phosphopeptides enrichment
  • Phosphorylation site identification
  • Global phosphor protein identification
• Palmitoylation
• Ubiquitination/SUMOylation
• Acetylation, methylation, and dimethylation
• Other chemical modifications (e.g., covalently bound irreversible molecule)

Protein identification

• Bottom-up LC MS/MS
• Multi-dimensional protein identification technology (MudPIT)
• IP and Co-IP protein identification
• Cross-linked protein identification
• Identification of proximity-labeled proteins (e.g., BioID)
• Contamination protein ID

Protein profiling

• Offline protein/peptide fractionation
• Stable isotopic labeling by amino acids in cell culture (SILAC)
• Label-free quantitation (LFQ)
• Multi-dimensional protein identification technology (MudPIT)
• TMT
• Data-independent acquisition (DIA)

Small-molecules quantitation

• MRM (multiple reaction monitoring)
• Biocrates p180 assay
• PK study
• Drug metabolite quantitation

Targeted proteomics

• PRM (parallel reaction monitoring)
• Data-independent acquisition (DIA) for cerebrospinal fluid (CSF)