LSM 880 Airyscan NLO/FCS Confocal Microscope
Joseph Wolenski, PhD
Yale Science Building, room C181C
About the instrument
Airyscan 32-channel ultra-sensitive area detector for superresolution imaging (140 nm in x, y at 488 nm) at very low laser power suitable for live-cell imaging with all common fluorescence labels.
Specifications
Laser lines:
Argon: 458, 488, 514 nm; 25 mW
Diode: 405 nm
DPSS: 561 nm
HeNe: 633 nm
Mai Tai DeepSee Tunable Laser: 690-1040 nm
ZEN 2.1 software with the following modules:
Z-stacks
Experiment Designer
Multi Channel
Image Analysis
Time Lapse
Temperature and CO2 controlled stage incubator for imaging live samples
Definite Focus
Point scanning photo stimulation (1P, 2P)
Spectral unmixing
Colocalization
Measurement
FCS extended
FLIM PicoQuant
HXP fluorescence illuminator and fluorescent filters for ocular viewing:
Filter Set 43: Alexa 546, Bodipy, Cy3, DsRed, dTomato, mOrange, Rhodamine
Filter Set 49: DAPI, Hoechst, BFP, Indo-1.
Filter Set 38: GFP, Alexa 488, CFP, FITC.
Objectives: fluorescence, brightfield, and DIC
EC Plan-Neofluar 10x/0.30 NA WD=5.2 mm
Plan-Apochromat 20x/0.8 NA WD=0.55 mm
Plan-Apochromat 40x/1.3 NA oil
C-Apochromat 40x/1.2 NA Water or Type W oil
Plan-Apochromat 63x/1.40 NA oil
Alpha Plan-Apochromat 100x/1.46 NA oil
Spectral detection using 32 channels PMTS (2) and ultra-sensitive GaAsP detector
BIG-2 GaAsP detector as NDD for 2P and FLIM detection
Rates
Rate for FY 24: $52/hour
Monthly billing is not capped as of August 1, 2021.