LSM 510 META Confocal Microscope
Joseph Wolenski, PhD
Yale Science Building, room C181B
About the instrument
Ideal for imaging thick fluorescently labeled specimens thanks to the ability to collect thin optical sections with minimal background fluorescence from out-of-focus structures.
Available to Yale researchers only
Specifications
Laser lines:
Argon: 458, 477, 488, 514 nm.
DPSS: 561 nm.
HeNe: 633 nm.
Chameleon (680-1080)
ZEN Blue software
Z-stacks
Tiles
Time Lapse
Image analysis
Spectral unmixing
Colocalization module
Measurement module
File format:
LSM, CZI, JPG, TIF
HXP fluorescence illuminator and fluorescent filters for ocular viewing:
Filter Set TRITC: Alexa 546, Bodipy, Cy3, DsRed, dTomato, Rhodamine
Filter Set 49: DAPI, Hoechst, BFP, Indo-1.
Filter Set 38: GFP, Alexa 488, CFP, FITC.
Objectives: Fluorescence, brightfield, and DIC
EC Plan-Neofluar 10x/0.30 NA WD=5.2 mm
Plan-Apochromat 20x/0.8 NA WD=0.55 mm
C-Apochromat 40x/1.2 NA water
Plan-Apochromat 63x/1.40 NA oil
Alpha Plan-Apochromat 100x/1.40 NA oil
Spectral detection using the META detector
Rates
Rates for FY24: $52/hour
Monthly billing is not capped as of August 1, 2021.