PCR purification

PCR purification is a 96-well plate format which is available for researchers who are experienced users of the facility and are confident in their methodology for PCR and primer preparation. Because the service is plate-based, every well gets processed.  Therefore; we have to charge for the entire submission, even if there are empty wells. We sequence and charge for all wells on the plate regardless of the results, unless it is a clear technical error on our part.

Submission requirements

You will submit your PCR template in one plate, and your primer will be provided separately.

• PCR product: 
  • to be submitted in 96-well plate format with each well containing 20 µl of 15-25 ng/µl of UNPURIFIED PCR product.
  • Please see our Plasticware Requirements page for appropriate plate submission types.
• Primer(s): 
  • to be submitted separately from the PCR products, in 1 of 2 formats: a single tube or a 96-well plate.

1. Tube format: Eppendorf tube with 384 µl of 4 µM primer. *ALL products will receive this 1 primer.
2. 96-well format: 5 µl of 4 µM primer/well. Primers should be oriented according to PCR plate layout.

Submission notes

Cap the plate firmly using the strip caps or sealing film and wrap each plate with parafilm. (Sealing film is preferable to strip caps.)

Be sure to clearly label each plate as either PCR plate or Primer plate (or Primer Tube), and label each order with a unique name.

Submissions should be dropped off with an order confirmation sheet (available to print after web ordering via our “Sample Submission” page).

Once we have purified your PCR product, we will then add your primer and sequence your PCR product. Your samples are sequenced by our standard sequencing protocol. If you wish to use another sequencing protocol, please let us know ahead of time.

Results will be posted in your Box account and labeled with the researcher code - plate name -and well-coordinate (RES CODE-plate name-A1-12, RES CODE-plate name-B1-12 etc). The researcher is responsible for holding the "key" to the well co-ordinates for sample identification.

For any questions or concerns, please contact the lab directly, 203-737-2566 or dnaseq@yale.edu.