PCR purification
Keck DNA Sequencing
300 George Street, Floor 2, Room 2127
About the service
PCR purification is a 96-well plate format which is available for researchers who are experienced users of the facility and are confident in their methodology for PCR and primer preparation. Because the service is plate-based, every well gets processed. Therefore; we have to charge for the entire submission, even if there are empty wells. We sequence and charge for all wells on the plate regardless of the results, unless it is a clear technical error on our part.
Available to Yale researchers & external researchers
Core website
PCR Purification
Standard turnaround time for PCR Purification is 1 business day. Yale users will receive priority over non-Yale users. If your samples are RUSH samples, please make us aware of this before we receive your submission.
Sample SubmissionSpecifications
You will submit your PCR template and primer separately. Please label each submission clearly.
- PCR product submission
- 20 µl containing 15-25 ng/µl of UNPURIFIED PCR product in a 96-well plate with clear wells.
- Primer(s) submission:
- 4uM primer to be submitted separately from the PCR products, in 1 of 2 formats: a single tube or a 96-well plate.
- If using one primer for all samples, you may submit in tube format: any tube containing 384 µl of 4 µM primer.
- If using multiple primers, put them in the appropriate wells of a 96-well plate corresponding to your sample plate layout. 96-well plate format: 5 µl of 4 µM primer per well.
Rates
As of 7/1/2024
| Service | Internal user | External for-profit user | External non-profit user |
|---|---|---|---|
| PCR purification & sequencing, per 96-well plate | $384.00 | $528.00 | $528.00 |
Submission notes
- Cap the plate firmly using the strip caps or sealing film and wrap each plate with parafilm. (Sealing film is preferable to strip caps.)
- Be sure to clearly label each plate as either PCR plate or Primer plate (or Primer Tube), and label each order with a unique name.
- Submissions should be dropped off with an order confirmation sheet (available to print after web ordering via our “Sample Submission” page).
- Once we have purified your PCR product, we will then add your primer and sequence your PCR product. Your samples are sequenced by our standard sequencing protocol. If you wish to use another sequencing protocol, please let us know ahead of time.
- Results will be posted in your Box account and labeled with the researcher code - plate name -and well-coordinate (RES CODE-plate name-A1-12, RES CODE-plate name-B1-12 etc). The researcher is responsible for holding the "key" to the well co-ordinates for sample identification.
How it works
Our PCR purification service is bead-based. We use Beckman Coulter's AMPure XP beads to perform our PCR cleanup.
Image is from the AMPure XP Instructions for Use
