Molecular and Serological Diagnostics
We test tissues, cells, feces and environmental samples for veterinary viruses, bacteria, parasites, and fungi by polymerase chain reaction (PCR) and reverse transcriptase-PCR (RT-PCR), and mouse and rat sera for viral antibodies.
About the core
Molecular diagnostics
We test tissues, cells, feces and environmental samples for veterinary viruses, bacteria, parasites, and fungi by polymerase chain reaction (PCR) and reverse transcriptase-PCR (RT-PCR).
The Yale IACUC requires biological contaminant testing (BCT) of samples that are proposed to be inoculated into rodents prior to use. This service is free of charge to Yale investigators.
Sample submission
Samples should be collected using aseptic technique and stored at -70C until delivery to BML B33. The following sample types are accepted:
- Cultured cells: 1,000,000 cells in a plastic tube with 1 ml of used media
- Tumors or tissues: 100 mg
- Feces: up to 6 mouse pellets per 1.5 ml tube
- Environmental samples: up to 4 synthetic swabs in a 1.5 ml tube
Include a submission form containing a COA or PO # and contact information with your sample(s). Email Susan Compton for a submission form or to inquire about available assays.
Serological diagnostics
We test mouse and rat sera for rodent viruses and Mycoplasma pulmonis.
Sample submission
Store samples at -20C until delivery to BML B33. The following sample quantities are requested. See table below for the number of tests involved in each type of request and, thus, the sample volume required.
- For 1-5 tests: submit 50 ul of undiluted serum or 500 ul of serum diluted 1:10 in saline
- For 6-12 tests: submit 100 ul of undiluted serum or 1 ml of serum diluted 1:10 in saline
Do not submit whole blood.
Include a submission form containing a COA or PO # and contact information with your sample(s). Email Susan Compton for a submission form or to inquire about available assays.
Rates
The Molecular and Serological Diagnostics Unit utilizes four services to provide investigator requests. Services and user rates are effective July 1, 2024.
| Service | Yale users | Non-Yale users |
|---|---|---|
PCR extraction (molecular) | $24.00 | $51.00 |
PCR test (molecular) | $20.00 | $44.00 |
IFA slides (not MPV) | - | $9.00 |
IFA slides (MPV only) | - | $21.00 |
Virology panel rates
Service requests involve a combination of services. In the table below, for each type of service request, the number of combined services is indicated, and the total cost of the service determined by the user rates above.
| Panel name | Services required by the test/panel | Yale user | Non-Yale user |
|---|---|---|---|
PCR- single PCR | 1 PCR extraction + 1 PCR test | $44.00 | $95.00 |
PCR-mouse relocation | 2 PCR extractions + 2 PCR tests | $88.00 | $190.00 |
PCR-fungal | 1 PCR extraction + 5 PCR tests | $124.00 | $271.00 |
PCR-BCT rat | 2 PCR extractions + 7 PCR tests | $188.00 | $410.00 |
PCR-BCT mouse | 2 PCR extractions + 11 PCR tests | $268.00 | $586.00 |
PCR-BCT combo | 2 PCR extractions + 12 PCR tests | $288.00 | $630.00 |
PCR-mouse quarantine | 3 PCR extractions + 29 PCR tests | $652.00 | $1,429.00 |
PCR-rat quarantine | 3 PCR extractions + 25 PCR tests | $572.00 | $1,253.00 |
IFA slides (not MPV) | Flat rate/slide | $0.00 | $9.00 |
IFA slides (MPV only) | Flat rate/slide | $0.00 | $21.00 |
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Primary contact
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