Genome editing using CRISPR/Cas9 technique

Genome editing in hESCs and iPSCs

The Yale hESC/iPSC Core provides services of single/a few nucleotide(s) replacement(s), knock-in and knock-out pieces of DNA in pluripotent cells using CRISPR/Cas9 technology. This can be done with in-house hESC or iPSC lines or custom-provided cell lines. 

Although we do accept live cells, we prefer frozen vials that have been confirmed to be negative for mycoplasma contamination. For cell lines derived from patients, they need to have been tested and found to be negative for HIV, Hepatitis B and Hepatitis C. 

The service takes 3 to 5 months to complete. This includes delivery of 3-4 clones, which are sequence-confirmed for right editing. 

Single/a few nucleotides replacement (s)

Gene knockout or deletion piece of genome

Insert reporter at specific locus in the genome.

 

Available to Yale researchers & external researchers

• Cell- & tissue-based technologies