CyTOF Facility
TAC s416
About the core
The CyTOF Helios analyzes cells labeled with stable heavy-metal isotopes using state-of-the-art time-of-flight atomic mass cytometry technology. Single-cell experimentation methods provide critical advances for research in cancer, cardiovascular biology, embryonic stem cells and development, hematopoietic stem cells and progenitors, immunity and infectious disease.
- Multi-parametric single-cell data, deep profiling
- Detect up to 42 parameters simultaneously in a single sample
- Detect more from limited samples
- Advantages over traditional flow cytometry: No background, no overlap between channels, no compensation issues
- Now available for tissue sections with imaging mass cytometry (IMC)
Reagents
In-house in our core we maintain:
- A repository of well-characterized metal conjugated antibodies
- Metal labeling reagents for conjugation of antibodies to these metals: 145Nd, 148Nd, 158Gd, 160 Gd, 164Dy, 165Ho, 171Yb, 173Yb
- Metal conjugated anti-fluorochrome antibodies (e.g. anti-FITC) that can be used with existing fluorescent reagents
- CyTOF Antibody Panel Guide
- Individual metal-conjugated individual antibodies (>500) and panel kits are available from Fluidigm
Available resources
- Cell surface staining protocol for CyTOF2 mass spectrometry
- Sample preparation using barcoding for CyTOF
- Data analysis and clustering algorithms of CyTOF data through the Bioinformatics Service at the Yale Center for Genome Analysis
- Useful web links for new users
Biosafety policies
All infectious, human, and non-human primate cells must be fixed before they are analyzed on the CyTOF. Schedule through LOTIS.
Publications
Recent publications from the Yale CyTOF facility
Boddupalli CS, Bar N, Kadaveru K, et al. Interlesional diversity of T cell receptors in melanoma with immune checkpoints enriched in tissue-resident memory T cells. JCI Insight. 2016;1(21):e88955. Published 2016 Dec 22. doi:10.1172/jci.insight.88955
Montgomery RR. High standards for high dimensional investigations. Cytometry A. 2016;89(10):886-888. doi:10.1002/cyto.a.22992
Yao Y, Welp T, Liu Q, et al. Multiparameter Single Cell Profiling of Airway Inflammatory Cells. Cytometry B Clin Cytom. 2017;92(1):12-20. doi:10.1002/cyto.b.21491
Vasquez JC, Huttner A, Zhang L, et al. SOX2 immunity and tissue resident memory in children and young adults with glioma. J Neurooncol. 2017;134(1):41-53. doi:10.1007/s11060-017-2515-8
Herndler-Brandstetter D, Shan L, Yao Y, et al. Humanized mouse model supports development, function, and tissue residency of human natural killer cells. Proc Natl Acad Sci U S A. 2017;114(45):E9626-E9634. doi:10.1073/pnas.1705301114
Publications for download
Yao Y, Liu R, Shin MS, et al. CyTOF supports efficient detection of immune cell subsets from small samples. J Immunol Methods. 2014;415:1-5. doi:10.1016/j.jim.2014.10.010 PDF
Strauss-Albee DM, Fukuyama J, Liang EC, et al. Human NK cell repertoire diversity reflects immune experience and correlates with viral susceptibility. Sci Transl Med. 2015;7(297):297ra115. doi:10.1126/scitranslmed.aac5722 PDF
Das R, Verma R, Sznol M, et al. Combination therapy with anti-CTLA-4 and anti-PD-1 leads to distinct immunologic changes in vivo. J Immunol. 2015;194(3):950-959. doi:10.4049/jimmunol.1401686 PDF
Available to Yale researchers & external researchers
Core Website
Sample submission form
Please contact us at CytofFacility@yale.edu to request sample preparation guidelines and a sample submission form.
Contact usContacts
203-785-7049
Primary contact
Biotechnology associate
Senior Administrative Assistant
To join CyTOF Users Group email list and for schedule of meetings
Rates
CyTOF analysis is available to Yale University investigators as a fee for service. Non-Yale users will be accommodated as possible. Please contact Dr. Konnikova.
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