In Vivo Imaging (IVI) Facility
David Gonzalez, MHS LAT
Facility ManagerBuilding: TAC, rooms S-614 and S-600
About the core
Multiphoton microscopy is an advanced fluorescence imaging technique that uses near-infrared pulsed lasers to excite fluorescent molecules deep into living, intact biological tissues with minimal cell damage. This research methodology has the advantage of direct visualization of 4D cellular arrangements and events that are often hard to deduce through sole use of in vitro assays and frozen sections.
The In Vivo Imaging (IVI) Facility core’s goal is to assist investigators in experimental design and implementation utilizing multiphoton microscopy. It serves as a clearinghouse for expertise and experience, being able to translate successful approaches to multiple projects and investigators. It also provides training for those who wish to learn surgical preparations and imaging acquisition at the facilities microscope and data analysis at the facilities image workstation using Imaris software (Oxford Instruments).
Available to Yale researchers only
Instruments/equipment
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LaVision Biotec TriMScope
An upright, laser scanning, multiphoton microscope with a dual output Coherent Discovery laser.
Available to Yale researchers only -
Microscope suite
Antechamber with a sink, lab bench, imaging platforms and dissecting microscope for specimen preparation and a dark imaging room that houses the microscope.
Available to Yale researchers only -
PC workstation with Imaris software (Oxford Instruments)
Post-acquisition image analysis.
Available to Yale researchers only
Policies
General
- To gain access to the facility and training, investigators must contact the manager, David Gonzalez, or the director, Ann Haberman.
- Instruments cannot be removed from the lab at any time except with staff authorization.
- Users of the facility should not store samples, solutions, or other materials within the facility unless authorized, and then only in designated areas. Any sample or solution left unlabeled will be discarded.
- Data stored in the Imaris workstations that is older than two months will be deleted without prior notice.
- Data stored on the microscope workstation that is older than six months will be deleted without prior notice if space is needed.
Training
- Training for surgical procedures and operation of the microscope is provided on a fee-for-service basis to investigators.
- Training can only be performed by facility staff.
- For more information on training, please contact David Gonzalez.
Computers
- Users are not allowed to download or install software on any of the computers without asking the staff. These computers are available exclusively for imaging samples, analysis and 3-4 dimensional reconstructions of pictures.
- Users should back up their files and delete their files from hard disks. Hard disks will be cleaned up on a regular basis without advance notice.
Rates
FY 27
| Service | Rate |
|---|---|
| 2P microscope, imaging independently | $65/hour |
| 2P microscope, imaging w/technician | $130/hour |
| 2P microscope training | $130/hour |
| Facility workstation use (Imaris) | $15/hour |
| Technician-assisted data analysis (Imaris) | $55/hour |
| Imaris software training | $55/hour |
| Skin preparation | $70/prep |
| Intestine preparation | $94/prep |
| Spleen preparation | $94/prep |
| Liver preparation | $94/prep |
| Calvarium preparation | $94/prep |
| Testicular preparation | $94/prep |
| Lung preparation | $94/prep |
| Popliteal lymph node preparation | $94/prep |
| Inguinal lymph node preparation | $94/prep |
Contacts
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