Other flow cytometry services
Yale Flow Cytometry Facility
Other services
Cytometry-related fee-for-service options & validated antibody panels
Many lines of hands-on services by facility staff are available. Staff can operate any analytic cytometer for you to acquire data on your behalf. Staff are also available to analyze data if needed. Charges for all services are cost-recovery for staff salary and hourly cost of any instruments used.
In addition, some forms of sample processing are offered with advance notice and discussion with the facility manager. Please contact Lesley Devine to ensure your project is appropriate and can be accommodated. Fees for these services can be found below in the rates section.
- PBMC isolation and cryopreservation
- Plasma or serum purification
- Luminex assay, data acquisition & analysis (with user-provided kits, charges are cost recovery for staff effort and machine usage)
- Immunofluorescence surface or intracellular cell staining, analytic data acquisition and analysis
The Flow Cytometry Core offers advanced antibody panels with high parameters specifically created for use on BD Symphony cytometers.
The panels are appropriate for peripheral blood, splenocytes, and some tumor micro environments.
Currently, the core provides four panels, see below. Reagents can be purchased individually by filling out downloadable forms that include a list of antibody specificities.
- Human T-cell panel (20 markers + viability)
- Human myeloid & B-cell panel (22 markers + viability)
- Murine panel (17 markers + viability)
- Mouse T-cell panel (in progress)
Please send your request to Ashraf Khalil at least one day in advance of pickup.
| For pickup at these times: | Send form to Ashraf by: |
|---|---|
| Mon 1-2:30 | Prior Friday |
| Wed 1-2:30 | Prior Tuesday |
Contact Ashraf Khalil for further technical details or Lesley Devine for general inquiries about lines of service involving sample preparation.
Please note that we are unable to provide reagents on short notice and can only provide them during limited windows of time with advance notice. Staff simply do not have the flexibility in their schedules to do otherwise.
Available to Yale researchers & external researchers
Rates
| Description | Fee |
|---|---|
| Luminex (core facility staff run assay, user provides the kit) | $498.00 |
| PBMC isolation and cryopreservation (for up to 20ml blood) | $100.00 |
| Serum/plasma purification | $55.00 |
| Cell surface staining (cost per sample) | $200.00 |
| Cell surface staining (additional sample) | $60.00 |
| Intracellular staining (cost per sample) | $260.00 |
More information
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trainingrequestguide-flowcytometry.pdf (2.42 MB)PPMS Training Request Guide
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Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration (OCR) and acidification (ECAR) are assessed with the Agilent Seahorse XF Pro 96. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. Changes in acidification are also observed simultaneously. An integrated Agilent BioTek Cytation5 imager correlates cell number via Hoechst nuclear stain to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS
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Instrument/equipment
Invitrogen Attune NXT Cell Analyzer
Analyze cells from tubes and plates with 4 lasers (violet, blue, yellow/green, red) and 14 detectors (6 violet, 2 blue, 3 yellow, 3 red).
Located within West Campus Flow Cytometry Facility -
Instrument/equipment
BD Symphony S6 Cell Sorter
Sort into 5 mL round tubes, 15 mL conical tubes, or plates (24-, 48-, and 96-well) with 5 lasers (UV, violet, blue, yellow/green, red) and 23 detectors (6 UV, 6 violet, 4 blue, 4 yellow/green, 3 red).
Located within West Campus Flow Cytometry Facility