Cell sorting: ThermoFisher BigFoot
The core's two BigFoot spectral cell sorters are located in TAC 552. The jet-in air sorter is encased in a Class II Biosafety Cabinet with an adjustable sash to maintain containments while allowing easy access to the nozzle and sample/sort areas, providing constant user protection. A dedicated aerosol management system (AMS), HEPA filter, and software provides containment warnings and increases flow if a clog is detected.
The Bigfoot cell sorter is gentle with fragile cells and can accommodate a range of nozzle sizes (70, 100, 120 and 150 um). It supports configurations with up to seven lasers (349 nm, 405 nm, 445 nm, 488 nm, 561 nm, 640 nm, 785 nm) and 55 detectors, delivering versatility for both standard fluorescence detection and spectral unmixing.
Multiple scatter options allow simultaneous standard and small-particle detection, multi-laser scatter detection, and polarization. The BigFoot allows input positions of 1.5 mL, 5 mL, and 15 mL tubes with automatic tube type sending and built-in crash detection. Several output collection options include 6-way sorting into 1.5 mL and 5 mL tubes, additionally 15 mL and 50 mL tubes, microwell plates up to 1,536 wells. Mixed output sorting is also available (i.e. 1.5 mL tubes for rare populations with 5-15 mL tubes for more abundant populations).
Location
TAC S552D