Location & contact — Tables
This BD FACSAriaII is is equipped with a biosafety cabinet, allowing one to sort unscreened human material. Learn more at the BD Biosciences website. For more detailed information or to schedule a sort, please contact Ewa Menet at 203-785-2299.
Location & contact
Amistad building, room 416
Contact: Ewa Menet, 203-785-2299
Tables
UV laser: 355 nm, 24 mWPMT | Bandpass | Long pass | Fluorochrome |
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A | 740/35 | 690 LP | BUV737 |
B | 450/50 | 410 LP | AlexaFluor 350, DAPI |
C | 379/29 | | BUV395 |
Violet laser: 405 nm, 200 mWPMT | Bandpass | Long pass | Fluorochrome |
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A | 710/50 | 685 LP | Qdot 705 |
B | 670/30 | 635 LP | Qdot 655 |
C | 610/20 | 600 LP | Qdot 605, BV605 |
D | 525/50 | 505 LP | AmCyan, Horizon V500 |
E | 450/50 | | Pacific Blue, BV421, Horizon V450 |
Filters for BV786 are also available (780/60 and 755LP).
Blue laser: 488 nm, 100 mWPMT | Bandpass | Long pass | Fluorochrome |
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A | 682/33 | 635 LP | PerCP-Cy5.5 |
B | 515/20 | 505 LP | FITC, AlexaFluor488, CFSE, GFP, YFP |
C | 488/10 | | SSC |
Green laser: 532 nm, 150 mWPMT | Bandpass | Long pass | Fluorochrome |
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A | 780/40 | 740 LP | PE-Cy7, PE-AF750 |
B | 710/50 | 685 LP | PE-Cy5.5 |
C | 660/40 | 640 LP | PE-Cy5, PE-AF647 |
D | 610/20 | 600 LP | AF594, PE-Texas Red, AF568, PI, Rhodamine-B |
E | 575/26 | | PE, DsRed, RFP, Pyronin-Y, PKH26 |
Red laser: 637 nm, 140 mWPMT | Bandpass | Long pass | Fluorochrome |
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A | 780/60 | 755 LP | APC-Cy7, Alexa Fluor 750, Alexa Fluor 780 |
B | 710/50 | 685 LP | APC-Cy5.5, Alexa Fluor 700 |
C | 660/20 | | APC, Alexa Fluor 647 |