Instrument/equipment Core/facility: Aberration-Corrected Electron Microscopy (ACEM) Core

Spectra Ultra Scanning Tranmission electron microscope

  1. Research Cores Directory
  2. Spectra Ultra Scanning Tranmission electron microscope

Shize Yang

Director

Spectra Ultra Scanning Tranmission electron microscope

Spectra Ultra Scanning Tranmission electron microscope will start working soon.
This webpage will be updated frequently with progress and be finalized when the tool is officially online.

Availability differs per offering

Spectra Ulra STEM microscope

Spectra Ultra crates

Spectra Ultra in crates at a warehouse

01/23/2025, 
Spectra Ultra in crates at a warehouse

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    In this subcore, parameters of cellular and tissue metabolic flux are assessed by incorporation of stable isotope labeled tracers (e.g., 13C, 15N, 2D) into metabolism. The CMC uses the targeted mass isotopomer multi-ordinate spectral analysis (MIMOSA) platform to interpret stable isotope labeling patterns for calculation of rates of discrete steps in glycolytic and mitochondrial metabolism. Such measurements can be used to identify differences in fuel usage (e.g., glycolysis vs. beta-oxidation), and non-oxidative (reductive carboxylation, anaplerosis and cataplerosis) contributions to the TCA cycle.  

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  2. Instrument/equipment

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  3. Services

    Islet isolation and function

    In this subcore, our technical expertise in isolating rodent islets including rats, mice and most recently ground squirrel islets, from the pancreas ex-vivo allows researchers to study the intact islets for their studies or to proceed with substrate stimulated insulin secretion functional studies using our BioRep Technologies Islet Perfusion Systems (8-channel and 12-channel) offered by our core. For non-rodent islet studies, human donor islet procurement assistance as well as unction studies are also offered.

     

    Located within Chemical Metabolism Core

    Rebecca Cardone, MS