Cytek Aurora Spectral cell analyzer
About the instrument
The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations, such as cells with high autofluorescence or low levels of expression of key biomarkers, regardless of assay complexity. [From the manufacturer website]
Available to Yale researchers & external researchers
Specifications
- 5 lasers: ultraviolet (UV), violet, blue, yellow/green, red
- 64 detectors: 16 UV, 16 violet, 14 blue, 10 yellow/green, 8 red
- Analyze from single tubes, 40-tube racks, or 96-well plates
Laser and detector guide
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Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration (OCR) and acidification (ECAR) are assessed with the Agilent Seahorse XF Pro 96. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. Changes in acidification are also observed simultaneously. An integrated Agilent BioTek Cytation5 imager correlates cell number via Hoechst nuclear stain to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS
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Instrument/equipment
Invitrogen Attune NXT cell analyzer
Analyze cells from tubes and plates with 4 lasers (violet, blue, yellow/green, red) and 14 detectors (6 violet, 2 blue, 3 yellow, 3 red).
Located within West Campus Flow Cytometry Facility -
Instrument/equipment
BD FACS LSR Fortessa X20
The Fortessa has 4 lasers, including V 405 nm, B 488 nm, and R 635 nm. It can detect 16 colors.
Located within Science Hill Flow Cytometry FacilityFreya Wencker, PhD