Mass spectrometry

Service only Shimadzu LCMS-8060 triple quadrupole liquid chromatograph mass spectrometer (LC-MS/MS). Triple quadrupole MS instruments allow for quantitation of compounds, collision-induced dissociation (CID), and multiple reaction monitoring (MRM) with high sensitivity. The UPLC system includs a fluidic system with two different solvents, three trays for up to 54 samples each (in GC vials), three UPLC columns (see list below) and a photo-diode array (UV/Vis) detector. 96 and 384 well plates can be used for screening projects; please reach out first.

Column 1: Shim-pack Scepter C18-120 1.9mum - 2.1 x 50mm
Column 2: Shim-pack Scepter C18-120 1.9mum - 2.1 x 100mm
Column 3: Shim-pack Scepter C8-120 1.9mum - 2.1 x 100mm

Different columns can be installed on demand.

Standard solvents available for LC separation:

Water (with 0.1% formic acid or 10mM NH₄Ac)
Methanol (with and w/o 0.1% formic acid or 10mM NH₄Ac)
Acetonitrile (with and w/o 0.1% formic acid or 10mM NH₄Ac)

Different solvents can be used on demand.

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Shimadzu 9030 QToF

Short name: 9030
Instrument location: KCL 3
Date of acquisition: January 2020

Shimadzu LCMS-9030 quadrupole time-of-flight high-performance liquid chromatograph mass spectrometer for high-resolution MS.

Service only high-resolution Q-tof MS instrument with an UPLC system, including a fluidic system with two different solvents, three trays for up to 54 samples each (in GC vials), three UPLC columns (see list below) and a photo-diode array (UV/Vis) detector. 96 and 384 well plates can be used for screening projects; please reach out first.

• Column 1: Shim-pack Scepter C18-120 1.9mum - 2.1 x 50mm
• Column 2: Shim-pack Scepter C18-120 1.9mum - 2.1 x 100mm
• Column 3: Shim-pack Scepter C8-120 1.9mum - 2.1 x 100mm

Different columns can be installed on demand.

Standard solvents available for LC separation:

• Water (with 0.1% formic acid or 10mM NH4Ac)
• Methanol (with and w/o 0.1% formic acid or 10mM NH4Ac)
• Acetonitrile (with and w/o 0.1% formic acid or 10mM NH4Ac)

Different solvents can be used on demand.

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Service only high-resolution QExactive Orbitrap FT-MS instrument with a Dionex 3000 UPLC system, including a fluidic system with up to four different solvents, three trays for up to 40 samples each (in 2 mL GC vials), one UPLC column, a UV/Vis photo-diode array (PDA), and a fluorescence (FL) detector.

The structural mass spectrometry extension on this instrument allows for recording of infrared spectra of mass-selected ions in the gas phase. Read more about the technique here.

Software for opening and converting .raw files: http://msfragger.nesvilab.org/tutorial_convert.html

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MALDI-ToF-MS Shimadzu AXIMA Confidence

Short name: MALDI
Instrument location: KCL 3
Date of acquisition: January 2020

AXIMA Confidence Linear/Reflectron MALDI-TOF mass spectrometer from Shimadzu.

 

Target plates available

AXIMA 384 well (2.8mm I.D.) plate - P/N: DE1580TA

Number of wells	Sample = 384 Calibrant = 0
Internal diameter of wells	Sample = 2.8 mm Calibrant = N/A

FlexiMass-SR48 steel targets - P/N: TO-431R00

Number of wells	Sample = 48 Calibrant = 3
Internal diameter of wells	Sample = 2.8 mm Calibrant = 2.2 mm

FlexiMass-DS disposable targets - P/N: TO-430R00

Number of wells	Sample = 48 Calibrant = 3
Internal diameter of wells	Sample = 2.8 mm Calibrant = 1.5 mm

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