BD FACS Aria Cell Sorter
About the instrument
- 4 lasers: violet, blue, yellow/green, red
- 14 detectors: 6 violet, 2 blue, 4 yellow/green, 2 red
- Sorting into: 5 milliliter (mL) round tubes, 15 ml conical tubes, or 24-, 48-, or 96-well plates
Available to Yale researchers & external researchers
Specifications
| Fluorophore | Filter | |||
| Violet | ||||
| 405 nm | BV786 | 780/60 | 750 LP | Super Bright 780 |
| BV711 | 710/50 | 685 LP | QDot 705, Super Bright 702 | |
| BV711 | 710/50 | 685 LP | QDot 705, Super Bright 702 | |
| BV650 | 670/30 | 635 LP | Super Bright 645 | |
| BV605 | 610/20 | 595 LP | Super Bright 600 | |
| BV605 | 610/20 | 595 LP | QDot 605 | |
| BV605 | 610/20 | 595 LP | PacOrange | |
| BV510 | 527/32 | 502 LP | AF430, Horizon V500, VioGreen, BV510, Pac Blue | |
| BV510 | 527/32 | 502 LP | AF430, Horizon V500, VioGreen, BV510, Pac Blue | |
| BV510 | 527/32 | 502 LP | AF430, Horizon V500, VioGreen, BV510, Pac Blue | |
| BV510 | 527/32 | 502 LP | AF430, Horizon V500, VioGreen, BV510, Pac Blue | |
| DAPI | 450/50 | BV421, Hoechst, Horizon 450, Alexa 405, CellTrace Violet, CFP, Zombie Violet, VioBlue, PacGreen, Sytox Blue, eFluor 450, Super Bright 436 | ||
| Blue | ||||
| 488 nm | BB700 | 710/50 | 690 LP | PerCp-Cy5-5, PerCP, Vio700 |
| FITC | 530/30 | 505 LP | BB515, AF 488, GFP, CFSE, Yo Pro1, Calcein, VioBright 515, Spark Blue 574, eFluor 520 | |
| Yellow/green | ||||
| 561 nm | PE-Cy7 | 780/60 | 750 LP | Pe Fire 810 |
| PE-Cy5 | 670/30 | 635 LP | 7AAD, mPlum, Pe Cy5.5, PE Fire 640, PE Fire 700 | |
| PE TxRed | 610/20 | 595 LP | mCherry(weak),mStrawberry, PECF594, PI,Alexa 594PE-Vio615, ECD, PE/Dazzle 594, PE eFluor610, Nova Fluor Yellow 610, PE-CF594 | |
| PE | 586/15 | 570 LP | PI, TRITC, DsRed, SYTOXOrange, Cy3, RFP, HRP, Calcein, Red Orange, mTangerine, Rhodamine, tdTomato, AF 555, Park YG570, Spark TG 581, Spark YG 593, eFluor 570 | |
| Red | ||||
| 633 nm | APC-Cy7 | 780/60 | 750 LP | APC-H7, RPE Cy7, Cy7, APC-AF750, APC CF780APC/FIRE750, APC FIRE 810, Spark NIR 718 |
| APC | 670/30 | AF 647, Cy5, AF633, DRAQ5, R PE Cy5, aFluor 660, ToPro3, APRK NIR 685 |
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Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration are assessed with the Agilent Seahorse XF Pro. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. An integrated Agilent BioTek Cytation5 imager correlates cell number to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS
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Instrument/Equipment
Invitrogen Attune NXT Cell Analyzer
Analyze cells from tubes and plates with 4 lasers (violet, blue, yellow/green, red) and 14 detectors (6 violet, 2 blue, 3 yellow, 3 red).
Located within West Campus Flow Cytometry Facility -
Instrument/Equipment
BD Symphony S6 Cell Sorter
Sort into 5 mL round tubes, 15 mL conical tubes, or plates (24-, 48-, and 96-well) with 5 lasers (UV, violet, blue, yellow/green, red) and 23 detectors (6 UV, 6 violet, 4 blue, 4 yellow/green, 3 red).
Located within West Campus Flow Cytometry Facility