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  1. Plate sequencing

    Plate sequencing Plate-based sequencing is a cost-effective and time-saving service recommended for those experienced in their methodology for template and primer preparation. Minimum charge for this service is 48 samples, but you may submit fewer. …

  2. Primer selection guidelines

    … guidelines Featured picture from here .     Back to Keck DNA Sequencing Standard primer sequences Outlined below … You can order these primers or custom primers via the Keck Biotechnology Resource Lab .  Primer Sequence (5'-3') …

  3. Plasticware requirements

    … Plasticware requirements Keck DNA Sequencing requires users to submit in plastics … caps, and 89004-284 tubes with green caps Site navigation Keck DNA Sequencing Cutoff time for Friday processing is 3 …

  4. PCR purification

    … XP Beads | PCR & NGS Library Cleanup Beckman Coulter links Keck DNA SequencingPCR PurificationCutoff time for Friday …

  5. Fragment analysis

    Fragment analysis Fragment Analysis Our 3730XL DNA Analyzer uses fluorescence-based detection to determine the size of DNA fragments.  Fragment analysis applicationsFragment analysis applications BAC Fingerprinting on 3730/3730xl Screen for Loss of …

  6. Direct sequencing of PCR products

    Direct sequencing of PCR products To obtain high quality sequencing data, it is very important that the PCR reaction is specific and strong. If the PCR product is a smear on an agarose gel, or more than one band is present, the likelihood of obtaining …

  7. Sanger sequencing

    … yield up to 1200 bases with 98% accuracy). Site navigation Keck DNA SequencingAlternative sequencing protocols In …

  8. Sanger sequencing troubleshooting guide

    Sanger sequencing troubleshooting guide It is part of our standard operating procedure that all sequence data generated at our Core facility are carefully inspected before they are made available to the end user.  We have implemented rigorous process …