Yale Lipidomics Innovation Core
Yeongho Kim, PhD
Director- yeongho.kim@yale.edu
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Booking via PPMS
Please register the project and order lipidomics services through PPMS.
9:00 AM–5:00 PM, Mon-Fri
About the core
The Yale Lipidomics Innovation Core (YLIC) offers lipidomics analytical services, including sample preparation, lipidomics data acquisition, data analysis, and data consulting. We use an untargeted LC-MS/MS approach and identify most major lipid species.
On request, we measure the absolute quantity of select lipid species in samples, including LPE, PE, ether PE, PG, PI, PS, cholesterol, cholesteryl ester, ceramide, glucosyl/galactosylceramide, lactosyl(hex2)ceramide, sphingomyelin, LPC, PC, ether PC, DG, ether DG, TAG, and ether TG.
We are actively incorporating lipidomics research and method development with Dr. Kallol Gupta of the Department of Cell Biology. We also work closely with other cores, including West Campus Analytical Core and Chemical Metabolism Core. Feel free to contact us to find the core that best suits your research.
Available to Yale researchers & external researchers
Guides for internal users
To view the guides, log in with your Yale ID at the prompt
Services
Sample preparation
- The core staff extracts lipids from user-provided samples.
- Cell pellets, in vitro assay materials, or tissue can be processed. If you are interested in using other types of samples, please contact us.
- The user can provide extracted lipids. For consistency and equipment maintenance, please contact us to confirm detailed information about your extraction protocols, solvents, and materials.
Data acquisition
- The core staff uses LC-MS/MS to acquire chromatograms and spectra of the lipidome. Experiment quality and sample quality will be assessed.
- If the sample quality issues are found, the user will be asked to provide another sample.
- If quantification of minor lipids is requested, multiple sample preparations and data acquisitions will be ordered.
Data analysis
- Lipids are identified and/or quantified (as requested) with high confidence using public, in-house, and/or commercial MS libraries.
Data consultation
- Users may request consultation to discuss data analysis and learn sample preparation and lipid analysis methodology.
Instruments / equipment
Our instruments include:
- Orbitrap Exploris 240 mass spectrometer
- Q Exactive UHMR Hybrid Quadrupole-Orbitrap mass spectrometer
- Vanquish Neo UHPLC system
- EASY-nLC 1200 system
West Campus Analytical Core is closely integrated with our core and expands our lipidomics-optimized instrumentation, including:
- Agilent 1290 InfinityII LC - Agilent 6546 qTOF
- Agilent 1290 UHPLC - Agilent 6550A qTOF
Chemical Metabolism Core provides services and facilities for metabolomics and targeted analysis.
Rates
External users, contact us for a quote.
Contacts
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Core/facility
Keck Biophysical Resource
As of 2026, the Keck Biophysical Resource will no longer be available for services except for NIDA investigators, who will still have access to some technologies.
Ewa Folta-Stogniew, PhD, MSc
Director (Retired) -
Core/facility
Yale Center for Molecular Discovery
We provide resources for assay development and high-throughput screening with small molecules and arrayed siRNA and sgRNA/CRISPR libraries in biochemical, cell-based and image-based assays (primarily 384-well format).
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Core/facility
Chemical Metabolism Core
Previously called the Islet, Oxygen consumption, Mass Isotopomer flux Core (IOMIC), the Chemical Metabolism Core offers targeted and untargeted metabolomics, stable isotope-based metabolic flux analysis, quantitative metabolic panels of central carbon metabolism and adjacent pathways, measurements of cellular bioenergetics, islet isolation and functional studies. Services include study assistance and data analysis.