Microbiology Service
About the service
The microbiology service performs aerobic and anaerobic bacterial culture, identification, optional antibiotic sensitivity and resistance, and fungal isolation from blood and tissue specimens from animals within the Yale vivarium. Examination of fur and fecal samples for ectoparasites and endoparasites, respectively, is available and can be requested.
Internal customers, please contact Gordon Terwilliger or Carmen Booth for more information.
External customers, please contact Carmen Booth for more information.
For external customers or consultation regarding interpretation of microbiology results, please contact Carmen Booth.
For serology or PCR diagnostic testing, please contact Sue Compton of the Department of Comparative Medicine's Molecular and Serological Diagnostics Laboratory.
A valid internal account (COA) is required before your samples can be accepted.
Available to Yale researchers & external researchers
Rates
Mouse Research Pathology Support is the only USP at the Yale School of Medicine providing pathologic phenotyping by comparative veterinary pathologists. In addition to gross and histologic evaluation of animal models, we also provide a variety of high-quality histology services tailored to individual projects.
Rates effective 1/1/25.
| Services | Unit | Yale research investigator | External research investigator |
|---|---|---|---|
| C&T technical time | per hour | $79.50 | $119.72 |
| M&P technical time | per hour | $79.00 | $115.00 |
| Pathologist | per hour | $140.00 | $204.50 |
| Necropsy: Research: Rodent (supplies only), for research animals only | per necropsy | $5.00 | $116.00 |
| Histology: Tissue processing only | per sample | $10.00 | $0 |
| Histology: Tissue processing and embedding | per sample | $12.25 | $35.00 |
| Histology: Tissue processing, embedding, sectioning, 1st H&E Slide | per sample | $21.00 | $65.50 |
| Histology: Cut unstained section from block (1-2 sections on one slide) | per slide | $3.50 | $14.75 |
| Histology: H&E slide | per slide | $5.25 | $69.00 |
| Histology: Special stains, non-silver stain only; no sectioning | per slide | $11.75 | $45.00 |
| Histology: Silver stain only; no sectioning | per slide | $21.00 | $83.00 |
| Histology: Frozen sectioning | per section | $21.00 | $400.00 |
| Microbiology: Aerobic culture, including Pseudomonas screening culture, urine cultures, cecal screening culture | per sample | $88.00 | $0 |
| Microbiology: Anaerobic culture | per sample | $130.00 | $0 |
| Microbiology: Antibiotic sensitivity -- group 1 | per sample | $84.00 | $0 |
| Microbiology: Antibiotic sensitivity -- group 2 | per sample | $84.00 | $0 |
| Microbiology: Blood culture | per sample | $145.00 | $0 |
| Microbiology: Diff Quik stain | per sample | $34.00 | $0 |
-
Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration (OCR) and acidification (ECAR) are assessed with the Agilent Seahorse XF Pro 96. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. Changes in acidification are also observed simultaneously. An integrated Agilent BioTek Cytation5 imager correlates cell number via Hoechst nuclear stain to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS
-
Instrument/equipment
Invitrogen Attune NXT Cell Analyzer
Analyze cells from tubes and plates with 4 lasers (violet, blue, yellow/green, red) and 14 detectors (6 violet, 2 blue, 3 yellow, 3 red).
Located within West Campus Flow Cytometry Facility -
Instrument/equipment
BD Symphony S6 Cell Sorter
Sort into 5 mL round tubes, 15 mL conical tubes, or plates (24-, 48-, and 96-well) with 5 lasers (UV, violet, blue, yellow/green, red) and 23 detectors (6 UV, 6 violet, 4 blue, 4 yellow/green, 3 red).
Located within West Campus Flow Cytometry Facility