Instrument/equipment Core/facility: West Campus Flow Cytometry Facility

BD Symphony A1 analyzer

WCFC Director

About the instrument

Offers premium high-end BD FACSymphony™ instrument technology scaled to fit on your benchtop
Provides flexibility to meet a broad spectrum of research needs from small particle research to 16-color immunophenotyping
Utilizes industry-standard BD FACSDiva™ Software for streamlined workflow from system setup to data acquisition and analysis
Analyze from single tubes or 96-well plates with 45 lasers (violet, blue, yellow/green, red) and 64 detectors (6 violet, 2 blue, 4 yellow/green, 3 red)

Available to Yale researchers only

	Fluorophore	Filter
Violet
405 nm	BV786	780/60	750 LP	Super Bright 780
	BV711	710/50	690 LP	QDot 705, Super Bright 702
	BV650	670/30	635 LP	Super Bright 645
	BV605	610/20	600 LP	Super Bright 600, QDot 605, PacORange
	BV480	470/14	470 LP	AF430, Horizon V500, VioGreen, BV510, Pac Blue
	BV421	431/28	410 LP	DAPI, Hoechst, Horizon 450, Alexa 405, CellTrace Violet, CFP, Zombie Violet, VioBlue, PacGreen, Sytox Blue, eFluor 450, Super Bright 436
Blue
488 nm	BB700	750/30	690 LP	PerCp-Cy5-5, PerCP, Vio700
	FITC	515/20	505 LP	BB515, AF 488, GFP, CFSE, Yo Pro1, Calcein, VioBright 515, Spark Blue 574, eFluor 520
Yellow -Green
561 nm	PE-Cy7	780/60	750 LP	Pe Fire 810
	PE-Cy5	670/30	635 LP	7AAD, mPlum, Pe Cy5.5, PE Fire 640, PE Fire 700
	PE-CF594	610/20	600 LP	mCherry(weak), mStrawberry, PE TxRed, PECF594, PI, Alexa 594PE-Vio615, ECD, PE/Dazzle 594, PE eFluor610, Nova Fluor Yellow 610
	PE	586/15	570 LP	PI, TRITC, DsRed, SYTOXOrange, Cy3, RFP, HRP, Calcein, Red Orange, mTangerine, Rhodamine, tdTomato, AF 555, Park YG570, Spark TG 581, Spark YG 593, eFluor 570
Red
637 nm	APC-Cy7	780/60	750 LP	APC-H7, RPE Cy7, Cy7, APC-AF750, APC CF780APC/FIRE750, APC FIRE 810, Spark NIR 718
	APC-R700	710/50	690/LP	AF700, APC-AF700, APC Cy5.5, AF 680, AF 600, Spark NIR 685
	APC	670/30	655 LP	AF 647, Cy5, AF633, DRAQ5, R PE Cy5, aFluor 660, ToPro3, APRK NIR 685