BD Symphony

The Facility has two identically configured BD Symphonies. Each is a six-laser system capable of detecting in 33 different channels. Please see the configuration below for details and view the Symphony resource page. Another way of viewing the configuration is using Fluorofinder website, a useful panel building tool listing reagents from multiple companies. You can go to fluorofinder.com and follow Tools--> Flow Cytometry Panel Builder-->> Select your Instrument (after choosing Yale and School of Medicine FACS Facility from the drop-down menu).

The Symphonies are equipped with a 561 nm yellow-green laser; this is different from the LSRIIs and Fortessas, which have 532 nm green lasers. You should expect your PE and PE-tandem dyes to appear brighter than they did before. Less antibody might be required to avoid extreme adjustments on the sensitive detectors of this model.

Some fluorescent proteins that were barely detectable with a 532 nm laser will appear significantly brighter with 561 excitation. mCherry, mKate, and mRaspberry will perform much better with this laser line.

The Facility offers remote Symphony training for experienced LSRII/Fortessa users. Whether you've trained on the instrument before the pandemic and are looking for a refresher, or you've just decided you want to start using our sensitive 6 laser cytometers, our training video will raise your confidence to use the Symphony. Please contact Jennifer Kelly for information about Symphony training. 

Both Symphonies are available 24/7 once you are an approved, trained user.

1) Symphony A in TAC S613 

2) Symphony B in Lab 2331 at 300 George St. This cytometer shares the room with a FACSAria sorter.