Namocell Pala cell sorter/cell dispenser
Fiona Hall, PhD
About the instrument
By combining proprietary microfluidics, flow cytometry, and liquid dispensing technologies, this benchtop cell sorter automates and simplifies single cell isolation across various applications: cell line development, cell engineering, single-cell genomics, monoclonal antibody development, and rare cell isolation such as circulating tumor cells (CTCs) and circulating fetal cells (CFCs). The Pala cell sorter and single-cell dispenser is affordable, easy to use, low-maintenance, and requires minimal training to operate. (From the manufacturer website)
Available to Yale researchers only
Specifications
2 lasers: blue and yellow/green
9 detectors: 5 blue and 4 yellow/ green
Sorting into: 96-well plates and Eppendorf tubes
| Filter | 488 nm | 561 nm |
|---|---|---|
| 527/32 | FITC | |
| 586/42 | Nile Red | PE |
| 625/15 | PE-Texas Red | mCherry |
| 692/80 | PerCP | PE-Cy5 |
| 783/56 | 488/783 | PE-Cy7 |
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Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration (OCR) and acidification (ECAR) are assessed with the Agilent Seahorse XF Pro 96. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. Changes in acidification are also observed simultaneously. An integrated Agilent BioTek Cytation5 imager correlates cell number via Hoechst nuclear stain to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS
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Instrument/equipment
BD LSR Fortessa X20
The Fortessa analyzer has 4 lasers, including V 405 nm, B 488 nm, YG 561 nm, and R 640 nm. It can detect 16 colors.
Located within Science Hill Flow Cytometry FacilityFreya Wencker, PhD
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Instrument/equipment
BD Symphony A1 analyzer
Analyze from single tubes or 96-well plates with 45 lasers (violet, blue, yellow/green, red) and 64 detectors (6 violet, 2 blue, 4 yellow/green, 3 red).
Located within West Campus Flow Cytometry Facility