Instrument/equipment Core/facility: Light Microscope Imaging Facility on Science Hill

Leica STELLARIS 8 TauSTED confocal microscope

Joseph Wolenski, PhD

Building:

Yale Science Building, room C181E

Available to Yale researchers only

Core website

Specifications

LED filter cubes:

405: DAPI

GFP, CFP/YFP

DsRed

Laser lines:

405 nm, DMOD 50 mW

White light laser (WLL): 440 to 790 nm, in 5 nm steps

Software:

LAS X 3D Visualization

LAS X Lightning Expert

LAS X Lightning STED Expert

LAS X Dye Finder

LAS X Environmental Control

LAS X MicroLab

Objectives:

HCX APO L 10x/0.30 W

HC FLUOTAR 25x/0.95 W VISIR

HC PL APO 40x/1.10 W CORR CS2

HCPL APO 86x/1.20 W motCORR STED W

HC PL APO 100x/1.40 oil STED

STED Module:

STELLARIS STED 660 laser

STELLARIS STED 775 laser

3D STED

TauSTED:  based on fluorescence lifetime

FALCON: FLIM, FLIM-FRET, FCS, Phasor

STELLARIS 8 FALCON

STELLARIS 8 FCS

Rates

Rate for FY24: $52/hour

Monthly billing is not capped as of August 1, 2021.